Quantitative analysis of prostate metabolites using 1H HR-MAS spectroscopy
Quantitative analysis of prostate metabolites using 1H HR-MAS spectroscopy
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DOI：
10.1002/mrm.20909
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发表时间：
2006-06-01
影响因子：
3.3
通讯作者：
Kurhanewicz, J
中科院分区：
文献类型：
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作者：
Swanson, MG;Zektzer, AS;Kurhanewicz, J
开发了一种使用H-1高分辨率魔角旋转（HR-MAS）光谱定量前列腺代谢物浓度的方法。测定主要前列腺代谢物和内部TSP标准的T-1和T-2弛豫时间（单位：毫秒），并用于优化11.7 T下的采集和重复时间（TR）。在1摄氏度，多胺（PA; T1（平均值）= 100 +/- 13，T-2平均值30.8 +/- 7.4）和柠檬酸盐（Cit; T1（平均值）= 237 +/- 39，T2（平均值）= 68.1 +/- 8.2）显示了最短的弛豫时间，而牛磺酸（Tau; T1（平均值）636 +/- 78 T2（平均值）331 +/- 71）和胆碱（Cho; T1（平均值）= 608 +/- 60，T2（平均值）= 393 +/- 81）显示最长的弛豫时间。使用代谢物和TSP峰面积以及组织和TSP的质量计算60个手术后组织的毫摩尔代谢物浓度。磷酸胆碱加甘油磷酸胆碱（PC+GPC）、总胆碱（tCho）、乳酸（Lac）和丙氨酸（Ala）浓度在前列腺癌中较高（[PC+GPC]（平均值）= 9.34 +/- 6.4% [tCho]（平均值）= 13.8 +/- 7.41 [Lac]（平均值）= 69.8 +/- 27.1，[Ala]（平均值）12.6 +/- 6.8）比健康腺体（[PC+GPC]（平均值）= 3.55 +/- 1.53，P < 0.01; [tChO]（平均值）= 7.06 +/- 2.36，P < 0.01; [LaC]（平均值）= 46.5 +/- 17.4，P < 0.01;[Ala]（平均值）8.63 ± 4.91，P = 0.051）和健康间质组织（[PC+GPC]（平均值）= 4.34 +/- 2.46，P < 0.01; [tChO]（平均值）= 7.04 +/- 3.10，P < 0.01; [LaC]（平均值）= 45.1 +/- 18.6，P < 0.01;[Ala]（平均值）6.80 ± 2.95，P < 0.01），而Cit和PA浓度在健康腺体组织中显著较高（[Cit]（平均值）= 43.1 +/- 21.2 [PAs]（平均值）= 18.5 +/- 15.6）（[Cit]（平均值）= 16.1 +/- 5.6，P < 0.01; [PAs]（平均值）= 3.15 +/- 1.81，P < 0.01）和前列腺癌组织（[Cit]（平均值）= 19.6 +/- 12.7，P < 0.01; [PAs]（平均值）= 5.28 +/- 5.44，P < 0.01）。在12小时内获得的系列光谱表明，与20 ℃相比，通过在VC下获得HR-MAS数据，含Cho的代谢物的降解最小化。
A method was developed to quantify prostate metabolite concentrations using H-1 high-resolution magic angle spinning (HR-MAS) spectroscopy. T-1 and T-2 relaxation times (in milliseconds) were determined for the major prostate metabolites and an internal TSP standard, and used to optimize the acquisition and repetition times (TRs) at 11.7 T. At 1 degrees C, polyamines (PAs; T1(mean) = 100 +/- 13, T-2mean 30.8 +/- 7.4) and citrate (Cit; T1(mean) = 237 +/- 39, T2(mean) = 68.1 +/- 8.2) demonstrated the shortest relaxation times, while taurine (Tau; T1(mean) 636 +/- 78 T2(mean) 331 +/- 71) and choline (Cho; T1(mean) = 608 +/- 60, T2(mean) = 393 +/- 81) demonstrated the longest relaxation times. Millimolal metabolite concentrations were calculated for 60 postsurgical tissues using metabolite and TSP peak areas, and the mass of tissue and TSP. Phosphocholine plus glycerophosphocholine (PC+GPC), total choline (tCho), lactate (Lac), and alanine (Ala) concentrations were higher in prostate cancer ([PC+GPC](mean) = 9.34 +/- 6.4% [tCho](mean) = 13.8 +/- 7.41 [Lac](mean) = 69.8 +/- 27.1, [Ala](mean) 12.6 +/- 6.8) than in healthy glandular ([PC+GPC](mean) = 3.55 +/- 1.53, P < 0.01; [tChO](mean) = 7.06 +/- 2.36, P < 0.01; [LaC](mean) = 46.5 +/- 17.4, P < 0.01; [Ala](mean) 8.63 +/- 4.91, P = 0.051) and healthy stromal tissues ([PC+GPC](mean) = 4.34 +/- 2.46, P < 0.01; [tChO](mean) = 7.04 +/- 3.10, P < 0.01; [LaC](mean) = 45.1 +/- 18.6, P < 0.01; [Ala](mean) 6.80 +/- 2.95, P < 0.01), while Cit and PA concentrations were significantly higher in healthy glandular tissues ([Cit](mean) = 43.1 +/- 21.2 [PAs](mean) = 18.5 +/- 15.6) than in healthy stromal ([Cit](mean) = 16.1 +/- 5.6, P < 0.01; [PAs](mean) = 3.15 +/- 1.81, P < 0.01) and prostate cancer tissues ([Cit](mean) = 19.6 +/- 12.7, P < 0.01; [PAs](mean) = 5.28 +/- 5.44, P < 0.01). Serial spectra acquired over 12 hr indicated that the degradation of Cho-containing metabolites was minimized by acquiring HR-MAS data at VC compared to 20 degrees C.