Development and characterization of marker MBH1 simultaneously tagging genes Pm21 and PmV conferring resistance to powdery mildew in wheat
Development and characterization of marker MBH1 simultaneously tagging genes Pm21 and PmV conferring resistance to powdery mildew in wheat
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同时标记基因 Pm21 和 PmV 的标记 MBH1 的开发和表征赋予小麦白粉病抗性
DOI：
10.1007/s11032-015-0385-3
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发表时间：
2015-10-01
影响因子：
3.1
通讯作者：
He, Huagang
中科院分区：
文献类型：
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作者：
Bie, Tongde;Zhao, Renhui;He, Huagang
小麦-簇毛麦易位系T6V#2S.6AL和T6V#4S.6DL分别是Pm21和PmV的携带者，在中国小麦品种中继续发挥抗白粉病作用。基于二穗短柄草与小麦族物种之间的共线性关系，获得了B.利用与Pm21的关键部分Stpk-V的同源物物理连锁的二穗粒设计引物并筛选共显性和稳定的标记。鉴定了标记6V#2S中Pm21和6V#4S中PmV的匿名标记MBH 1。它还区分了小麦6AS和6DS来源的同源等位基因MBH 1。序列长度比较显示6AS 6DS 6V#2S 6V#4S的降序。以扬麦18号（T6V#2S.6AL）/扬麦19和扬麦16号/扬麦22（T6V#4S.6DL）为材料，对F_2进行了分离分析。MBH 1检测到分离群体中Pm21和PmV的纯合性。结果还表明，T6V#2S.6AL比T6V#4S.6DL更易透射。利用MBH 1基因再次确认了扬麦21、珍麦9号和扬麦97 G59的抗白粉病基因的同一性。这三种病毒的耐药供体均为T6V#2S.6AL，这表明已发表的家系不正确。NAU 419-Y15中的Pm21也被MBH 1标记。MBH 1是一个广泛适用的标记，可用于选择和区分携带Pm21和PmV的易位染色体，在中国继续有效。
Wheat-Haynaldia villosa translocations T6V#2S.6AL and T6V#4S.6DL, carriers of Pm21 and PmV, respectively, continue to contribute powdery mildew resistance in wheat varieties in China. Based on colinearity between Brachypodium distachyon and Triticeae species, genomic sequences from gene intervals of B. distachyon physically linked to a homolog of Stpk-V, a key part of Pm21, were used to design primers and screen for codominant and stable markers. An anonymous marker, MBH1, tagging both Pm21 in 6V#2S and PmV in 6V#4S was identified. It also distinguished wheat 6AS- and 6DS-derived homoeoalleles of MBH1. Sequence length comparisons showed a decreasing order of 6AS > 6DS > 6V#2S > 6V#4S. F2 segregation analyses were conducted on crosses Yangmai 18 (T6V#2S.6AL)/Yangmai 19 and Yangmai 16/Yangmai 22 (T6V#4S.6DL). MBH1 detected homozygosity of both Pm21 and PmV in segregating populations. The results also showed that T6V#2S.6AL is more transmittable than T6V#4S.6DL. Using MBH1, we reconfirmed the identities of powdery mildew resistance genes in Yangmai 21, Zhenmai 9, and Yangmai 97G59. The resistance donor to all three was T6V#2S.6AL, indicating that the published pedigrees were not correct. Pm21 in NAU419-Y15, an intercalary translocation carrying Pm21, was also tagged by MBH1. MBH1 is a broadly applicable marker for selecting and distinguishing the translocation chromosomes carrying Pm21 and PmV, which continue to be effective in China.