Sol-gel-immobilized Tris(2,2′-bipyridyl)ruthenium(II) electrogenerated chemiluminescence sensor for high-performance liquid chromatography
Sol-gel-immobilized Tris(2,2′-bipyridyl)ruthenium(II) electrogenerated chemiluminescence sensor for high-performance liquid chromatography
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DOI：
10.1016/j.aca.2004.06.076
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发表时间：
2005-06-13
影响因子：
6.2
通讯作者：
Lee, WY
中科院分区：
文献类型：
--
作者：
Choi, HN;Cho, SH;Lee, WY
将溶胶-凝胶固定化三（2，2 '-联吡啶）钌（II）[Ru（bpy）（3）（2+）]电致化学发光（ECL）传感器用于反相高效液相色谱（HPLC）法测定人尿样中的吩噻嗪衍生物（丙嗪、氯丙嗪、三氟丙嗪、硫利达嗪和三氟拉嗪）和红霉素。在该方法中，Ru（bpy）（3）（2+）被固定在溶胶-凝胶法制备的二氧化钛（TiO 2）-Nation纳米复合膜上的双铂电极。该方法消除了将Ru（bPY）（3）（2+）试剂输送到光电倍增管前面的反应/观察区中所需的额外泵，因为固定化的Ru（bpy）（3）（2+）通过相对于Ag/AgCl（3 M NaCl）参比电极的+1.3 V的施加电势在电极表面上再循环。所得分析性能，如检测限，工作范围，灵敏度和测量精度略差于传统的柱后Ru（bpy）（3）（2+）添加方法。吩噻嗪衍生物和红霉素的检测限低，无明显干扰，表明该方法适用于生物体液中吩噻嗪衍生物和红霉素的测定。(c)2004 Elsevier B. V.保留所有权利。
The sol-gel-immobilized Tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)(3)(2+)] electrogenerated chemiluminescence (ECL) sensor was applied to the reversed-phase high-performance liquid chromatography (HPLC) determination of phenothiazine derivatives (promazine, chlorpromazine, triflupromazine, thioridazine, and trifluoperazine) and erythromycin in human urine samples. In this method, Ru(bpy)(3)(2+) was immobilized in sol-gel-derived titania (TiO2)-Nation nanocomposite films coated on a dual platinum electrode. This method eliminates an extra pump needed for the delivery of Ru(bPY)(3)(2+) reagent into a reaction/observation zone in front of photomultiplier tube because the immobilized-Ru(bpy)(3)(2+) is recycled on the electrode surface by an applied potential at + 1.3 V versus Ag/AgCl (3 M NaCl) reference electrode. The resulting analytical performances such as detection limit, working range, sensitivity, and measurement precision were slightly worse than those obtained with the conventional post-column Ru(bpy)(3)(2+) addition approach. The lack of significant interferences and the low detection limits for phenothiazine derivatives and erythromycin indicate that the proposed HPLC-Ru(bPY)(3)(2+) ECL detection method is suitable for the determination of those compounds in biological fluids. (c) 2004 Elsevier B.V. All rights reserved.