GALANIN ANTISENSE OLIGONUCLEOTIDES REDUCE GALANIN LEVELS IN DORSAL-ROOT GANGLIA AND INDUCE AUTOTOMY IN RATS AFTER AXOTOMY
GALANIN ANTISENSE OLIGONUCLEOTIDES REDUCE GALANIN LEVELS IN DORSAL-ROOT GANGLIA AND INDUCE AUTOTOMY IN RATS AFTER AXOTOMY
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DOI：
10.1073/pnas.91.26.12540
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发表时间：
1994-12-20
影响因子：
11.1
通讯作者：
HOKFELT, T
中科院分区：
文献类型：
--
作者：
JI, RR;ZHANG, Q;HOKFELT, T
Antisense (AS) oligonucleotides (ONs) to galanin (GAL) were applied to the proximal end of a transected sciatic nerve, allowing their cellular uptake and transport into injured axons. GAL expression in dorsal root ganglia and self-mutilation behavior (autotomy) were then studied. AS-ONs with phosphorothioate or allyl modifications significantly suppressed the axotomy-induced increase in GAL levels, as demonstrated by immunohistochemistry and exaggerated autotomy behavior, whereas no significant effect on GAL mRNA levels could be demonstrated with in situ hybridization. Allyl-ONs were more effective than phosphorothioate-ONs. An AS-ON with three base mismatches did not induce any of the above effects. These results support the view that the inhibition of arotomy-induced GAL up-regulation is related to autotomy.
