Zonarol Protected Liver from Methionine- and Choline-Deficient Diet-Induced Nonalcoholic Fatty Liver Disease in a Mouse Model.
Zonarol Protected Liver from Methionine- and Choline-Deficient Diet-Induced Nonalcoholic Fatty Liver Disease in a Mouse Model.
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DOI：
10.3390/nu13103455
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发表时间：
2021-09-29
期刊：
影响因子：
5.9
通讯作者：
Yamada S
中科院分区：
文献类型：
--
作者：
Han J;Guo X;Koyama T;Kawai D;Zhang J;Yamaguchi R;Zhou X;Motoo Y;Satoh T;Yamada S
非酒精性脂肪性肝病（NAFLD）是最常见的肝脏疾病之一，没有批准的治疗方法。Zonarol是一种从褐藻中提取的提取物，已被证明具有抗炎和抗氧化作用。在这项研究中，我们研究了zonarol在蛋氨酸和胆碱缺乏（MCD）饮食诱导的NAFLD小鼠进展中的作用。在用佐那洛尔口服治疗后，与对照组（CG）小鼠相比，在佐那洛尔组（ZG）小鼠中观察到较轻的体重。ZG小鼠NAFLD评分低于CG小鼠。ZG小鼠的肝脏和血清脂质水平也较低，脂质代谢相关因子的表达减少。此外，与CG小鼠相比，ZG小鼠表现出更少的脂质沉积、更少的炎性细胞浸润和更低的炎性细胞因子水平。此外，与CG小鼠相比，8-羟基-20-脱氧鸟苷（8-OHdG）阳性肝细胞的数量以及肝脏和血清硫代巴比妥酸反应物质（TBARS）的水平显著降低。核因子红细胞2相关因子2（Nrf 2）及其上游和下游分子的表达水平在ZG小鼠中发生变化。佐那洛尔可能通过减轻炎症反应、氧化应激、改善脂质代谢等途径阻止NAFLD的进展。Nrf 2信号通路可能在这些效应中起重要作用。
Nonalcoholic fatty liver disease (NAFLD) is one of the most common liver diseases with no approved treatment. Zonarol, an extract from brown algae, has been proven to have anti-inflammatory and antioxidant effects. In this study, we investigated the role of zonarol in the progression of methionine- and choline-deficiency (MCD) diet-induced NAFLD in mice. After oral treatment with zonarol, a lighter body weight was observed in zonarol group (ZG) mice in comparison to control group (CG) mice. The NAFLD scores of ZG mice were lower than those of CG mice. Hepatic and serum lipid levels were also lower in ZG mice with the reduced expression of lipid metabolism-related factors. Furthermore, ZG mice showed less lipid deposition, less inflammatory cell infiltration and lower inflammatory cytokine levels in comparison to CG mice. Moreover, the numbers of 8-hydroxy-20-deoxyguanosine (8-OHdG)-positive hepatocytes and levels of hepatic and serum thiobarbituric acid reactive substances (TBARS) were significantly lower in comparison to CG mice. The expression levels of nuclear factor erythroid 2 related factor 2 (Nrf2), as well as its upstream and downstream molecules, changed in ZG mice. Zonarol could prevent the progression of NAFLD by decreasing inflammatory responses, oxidative stress and improving lipid metabolism. Meanwhile the Nrf2 pathway may play an important role in these effects.