High macro-collinearity between Crassostrea angulata and C. gigas genomes was revealed by comparative genetic mapping with transferable EST-SNP markers
High macro-collinearity between Crassostrea angulata and C. gigas genomes was revealed by comparative genetic mapping with transferable EST-SNP markers
复制标题
利用可转移EST-SNP标记的比较遗传作图揭示了角长牡蛎和巨牡蛎基因组之间高度的宏观共线性
DOI：
10.1016/j.aquaculture.2021.737183
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发表时间：
2021-07-23
期刊：
影响因子：
4.5
通讯作者：
Yu，Hong
中科院分区：
文献类型：
--
作者：
Wang，Jiulong;Li，Qi;Yu，Hong
Comparative genomics has become an important strategy for transferring genetic information from a well-characterized model species to one that is less described and can facilitate in functional gene mining and genomic evolution.Crassostrea angulataandCrassostrea gigasare closed related species that they can hybridize to produce fertile offspring, andC. gigashas accumulated a considerable number of genetic and genomic resources whileC. angulatalags far behind. In the current study, 684 single nucleotide polymorphism (SNP) markers developed fromC. gigasexpressed sequence tags (ESTs) were used for transferability test inC. angulata, and 635 loci were successfully amplified. Then a genetic linkage map ofC. angulatawas for the first time constructed using these transferable SNP markers. A total of 988 transferable SNP markers were used for polymorphism screening in a mapping family, and 273 loci were distributed on the genetic map which was composed of 10 linkage groups and estimated 1040.29 cM in total length. The average spacing between markers was 3.96 cM. By comparing the shared EST-SNP marker locations on homologous linkage groups, comparative mapping analysis between theC. angulatagenetic map and an integrated map ofC. gigasrevealed putative macro-collinearity in the two oyster genomes with little difference in markers order. These transferable SNP markers could be applied in genetic diversity and population structure studies, and the genetic map would be useful for quantitative trait loci mapping and genetic improvement through marker-assisted selection forC. angulata.
