Genes encoding a group of related small secreted proteins from the gut of Hessian fly larvae [Mayetiola destructor (Say)]
Genes encoding a group of related small secreted proteins from the gut of Hessian fly larvae [Mayetiola destructor (Say)]
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DOI：
10.1111/j.1744-7917.2006.00102.x
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发表时间：
2006-10-01
期刊：
影响因子：
4
通讯作者：
Wilde, Gerald E.
中科院分区：
文献类型：
--
作者：
Chen, Ming-Shun;Liu, Xiang;Wilde, Gerald E.
一组相关基因已被分离和特点的黑森蝇幼虫[Mayetiola destructor（Say）]的肠道。该组中的成员似乎编码在N-末端具有分泌信号肽的蛋白质。成熟的推定蛋白质是小的，酸性蛋白质，计算的分子量为14.5至15.3 kDa，等电点为4.56至4.88。北方印迹分析表明，这些基因主要表达于黑森蝇幼虫和蛹的肠道中。两个相关基因，G10 K1和G10 K2被分离为串联重复序列。这两个基因都含有三个外显子和两个内含子。内含子/外显子边界在氨基酸编码方面是保守的，这表明它们是由基因复制引起的。事实上，这组克隆在肠道cDNA文库中的频率高于编码消化酶的总cDNA克隆的频率，这表明这组蛋白质可能在该昆虫的肠道生理学中发挥重要作用。然而，这些蛋白质的确切功能是已知的，因为没有序列相似性可以确定这些蛋白质和任何已知的序列在公共数据库中使用标准方法。
A group of related genes has been isolated and characterized from the gut of Hessian fly larvae [Mayetiola destructor (Say)]. Members in this group appear to encode proteins with secretary signal peptides at the N-terminals. The mature putative proteins are small, acidic proteins with calculated molecular masses of 14.5 to 15.3 kDa, and isoelectric points from 4.56 to 4.88. Northern blot analysis revealed that these genes are expressed predominantly in the gut of Hessian fly larvae and pupae. Two related genes, G10K1 and G10K2, were isolated as tandem repeats. Both genes contain three exons and two introns. The intron/exon boundaries were conserved in terms of amino acid encoding, suggesting that they arose by gene duplication. The fact that the frequency of this group of clones in a gut cDNA library higher than that of total cDNA clones encoding digestive enzymes suggested that this group of proteins may perform an important function in the gut physiology of this insect. However, the exact functions of these proteins are as yet known since no sequence similarity could be identified between these proteins and any known sequences in public databases using standard methods.