Analysis of breath volatile organic compounds as a noninvasive tool to diagnose nonalcoholic fatty liver disease in children
Analysis of breath volatile organic compounds as a noninvasive tool to diagnose nonalcoholic fatty liver disease in children
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DOI：
10.1097/meg.0b013e3283650669
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发表时间：
2014-01-01
影响因子：
2.1
通讯作者：
Dweik, Raed
中科院分区：
文献类型：
--
作者：
Alkhouri, Naim;Cikach, Frank;Dweik, Raed
非酒精性脂肪肝（NAFLD）是儿童肥胖症最常见的并发症之一。我们的目的是调查的协会呼吸挥发性有机化合物与NAFLD的诊断在children.MethodsPatients进行了筛选与腹部超声评估NAFLD。呼气收集和分析每协议使用选择性离子流管质谱（SIFT-MS）。ResultsSixty例患者被纳入研究（37例NAFLD和23例正常肝脏）。所有儿童均超重或肥胖。平均年龄为14.12.8岁，50%为女性。NAFLD患者与正常肝脏患者的SIFT-MS结果在超声上的比较显示了超过15种化合物浓度的差异。一组四种挥发性有机化合物可以以良好的准确度识别NAFLD的存在（训练集中的受试者工作特征曲线下的面积为0.913，验证集中的面积为0.763）。与正常肝脏组相比，NAFLD组的呼吸异戊二烯、丙酮、三甲胺、乙醛和戊烷显著较高（异戊二烯14.7 ppb对8.9 ppb;丙酮71.7 ppb对36.9 ppb;三甲胺5.0 ppb对3.2 ppb;乙醛35.1 ppb对26.0 ppb;戊烷13.3 ppb对8.8 ppb，P
ObjectiveNonalcoholic fatty liver disease (NAFLD) is one of the most common complications of childhood obesity. Our objective was to investigate the association of breath volatile organic compounds with the diagnosis of NAFLD in children.MethodsPatients were screened with an ultrasound of the abdomen to evaluate for NAFLD. Exhaled breath was collected and analyzed per protocol using selective ion flow tube mass spectrometry (SIFT-MS).ResultsSixty patients were included in the study (37 with NAFLD and 23 with normal liver). All children were overweight or obese. The mean age was 14.12.8 years and 50% were female. A comparison of the SIFT-MS results of patients with NAFLD with those with normal liver on ultrasound revealed differences in concentration of more than 15 compounds. A panel of four volatile organic compounds can identify the presence of NAFLD with good accuracy (area under the receiver operating characteristic curve of 0.913 in the training set and 0.763 in the validation set). Breath isoprene, acetone, trimethylamine, acetaldehyde, and pentane were significantly higher in the NAFLD group compared with normal liver group (14.7 ppb vs. 8.9 for isoprene; 71.7 vs. 36.9 for acetone; 5.0 vs. 3.2 for trimethylamine; 35.1 vs. 26.0 for acetaldehyde; and 13.3 vs. 8.8 for pentane, P