Detection of the human T cell lymphoma virus p19 in cells of some patients with cutaneous T cell lymphoma and leukemia using a monoclonal antibody
Detection of the human T cell lymphoma virus p19 in cells of some patients with cutaneous T cell lymphoma and leukemia using a monoclonal antibody
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使用单克隆抗体检测部分皮肤T细胞淋巴瘤和白血病患者细胞中的人T细胞淋巴瘤病毒p19
DOI：
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发表时间：
1981
影响因子：
15.3
通讯作者：
R. Gallo
中科院分区：
文献类型：
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作者：
M. Robert;F. Ruscetti;L. Posner;B. Poiesz;R. Gallo
已经开发出一种对从人类肿瘤T细胞中分离的C型逆转录病毒（HTLV）的内部p19蛋白具有特异性的单克隆抗体。其特异性已被放射免疫沉淀和碘化HTLV蛋白的亲和层析所证实。通过间接免疫荧光，该抗体仅识别HTLV产生细胞。对皮肤T细胞淋巴瘤和白血病以及其他类型的淋巴瘤和白血病患者的细胞检查表明，HTLV p19表达是罕见的。该单克隆抗体将有助于确定HTLV的天然储库，可能在成熟T细胞肿瘤的一个子集中。
A monoclonal antibody specific for the internal p19 protein of a type-C retrovirus (HTLV) isolated from human neoplastic T cells has been developed. Its specificity has been shown by radioimmune precipitation and by affinity chromatography of iodinated HTLV proteins. By indirect immune fluorescence this antibody recognizes only HTLV-producing cells. Examination of cells from patients with cutaneous T cell lymphomas and leukemias and with other types of lymphomas and leukemias indicated that HTLV p19 expression is rare. The monoclonal antibody will be useful in determining the natural reservoir of HTLV, possibly in a subset of mature T cell neoplasias.