JNK2-dependent regulation of SIRT1 protein stability
JNK2-dependent regulation of SIRT1 protein stability
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DOI：
10.4161/cc.7.19.6799
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发表时间：
2008-10-01
期刊：
影响因子：
4.3
通讯作者：
Milner, Jo
中科院分区：
文献类型：
--
作者：
Ford, Jack;Ahmed, Shafiq;Milner, Jo
哺乳动物SIRT1是一种NAD依赖性脱乙酰酶，在维持体内平衡和细胞存活中起关键作用。SIRT1蛋白水平升高在癌症中是明显的，其中SIRT1可以作为癌症特异性生存因子发挥作用。在这里，我们证明了在人类细胞中SIRT1蛋白的升高不是由于SIRT1 mRNA水平的增加，而是反映了SIRT1蛋白的稳定性。RNAi介导的JNK2耗竭使SIRT1蛋白的半衰期从> 9小时减少到> 10小时。
Mammalian SIRT1 is an NAD-dependent deacetylase with critical roles in the maintenance of homeostasis and cell survival. Elevated levels of SIRT1 protein are evident in cancer in which SIRT1 can function as a cancer-specific survival factor. Here we demonstrate that elevated SIRT1 protein in human cells is not attributable to increased SIRT1 mRNA levels but, instead, reflects SIRT1 protein stability. RNAi-mediated depletion of JNK2 reduced the half-life of SIRT1 protein from > 9 h to