Reversible alteration of hepatic messenger RNA species for peroxisomal and non-peroxisomal proteins induced by the hypolipidaemic drug Wy-14,643.
Reversible alteration of hepatic messenger RNA species for peroxisomal and non-peroxisomal proteins induced by the hypolipidaemic drug Wy-14,643.
复制标题
降血脂药物 Wy-14,643 诱导过氧化物酶体和非过氧化物酶体蛋白的肝脏信使 RNA 种类发生可逆改变。
DOI：
10.1042/bj2140879
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Roy,AK
中科院分区：
文献类型：
--
作者：
Chatterjee,B;Demyan,WF;Lalwani,ND;Reddy,JK;Roy,AK
当雄性大鼠喂食含有0.1% Wy-14，643 [（4-氯-6-（2，3-亚甲基）-2-嘧啶硫基]乙酸）（一种强效降脂药）的饲料时，观察到肝实质细胞中过氧化物酶体广泛增殖。该药物还导致编码Mr为77000、61000、43000和31000的四种蛋白质的mRNA种类的浓度显著增加，并且编码Mr为25000、24000和19000的蛋白质的三种mRNA种类的浓度类似地降低。特异性免疫沉淀研究将Mr 19000、43000和77000的蛋白质分别鉴定为α 2u-球蛋白、3-酮脂酰-CoA硫解酶（EC 2.3.1.16）和烯酰-CoA水合酶（EC 4.2.1.17）。比较的Mr值表明，61000-和31000-Mr的蛋白质可能相当于两个额外的过氧化物酶，即过氧化氢酶（Mr 61000）和尿酸酶（Mr 31000）。编码25000-和24000-Mr蛋白质的mRNA种类的身份目前尚不清楚。
Extensive peroxisomal proliferation in the hepatic parenchymal cells was observed when male rats were given a diet containing 0.1% Wy-14,643 [(4-chloro-6-(2,3-xylidino)-2-pyrimidinylthio]acetic acid), a potent lipid-decreasing drug. This drug also caused a marked increase in the concentrations of the mRNA species coding for four proteins with Mr 77000, 61000, 43000 and 31000, and a similar decrease in the concentrations of three mRNA species coding for proteins of Mr 25000, 24000 and 19000. Specific immunoprecipitation studies identified the proteins of Mr 19000, 43000 and 77000 as alpha 2u-globulin, 3-ketoacyl-CoA thiolase (EC 2.3.1.16) and enoyl-CoA hydratase (EC 4.2.1.17) respectively. Comparisons of the Mr values suggest that the 61000- and 31000-Mr proteins may be equivalent to two additional peroxisomal enzymes, namely catalase (Mr 61000) and uricase (Mr 31000). The identity of the mRNA species coding for the 25000- and 24000-Mr proteins is at present unknown.