Methods to Study Pulmonary Dendritic Cell Migration
Methods to Study Pulmonary Dendritic Cell Migration
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DOI：
10.1007/978-1-60761-421-0_24
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Randolph, Gwendalyn J.
中科院分区：
文献类型：
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作者：
Jakubzick, Claudia;Randolph, Gwendalyn J.
在感染、过敏和疫苗接种过程中，树突状细胞从呼吸道到淋巴结的迁移是呼吸道免疫发展中的一个关键事件。通过对材料的司法选择，有两种方法可以研究树突状细胞在不引起炎症的情况下向纵隔淋巴结的迁移：经呼吸道注射荧光OVA和乳胶珠。我们的方案描述了如何标记和跟踪从呼吸道和肺到纵隔淋巴结的肺树突状细胞，并揭示了如何避免陷阱和次优分析。
Dendritic cell migration from the airway to the lymph nodes is a key event in the development of airway immunity during infection, allergy, and vaccination. With judicial selection of materials, there are two approaches to study dendritic cell migration to the mediastinal lymph nodes Without the induction Of inflammation: airway administration of fluorescent OVA and latex beads. Our protocol describes how to label and track Pulmonary dendritic cells from the airway and lung to the mediastinal lymph nodes and reveals how to avoid pitfalls and suboptimal assays.