Development of Method to Determine the Concentration of Alkali-Soluble Lignin using Coomassie Brilliant Blue G-250
Development of Method to Determine the Concentration of Alkali-Soluble Lignin using Coomassie Brilliant Blue G-250
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DOI：
10.15376/biores.11.3.6137-6150
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发表时间：
2016-05
期刊：
影响因子：
1.5
通讯作者：
Fangfang Wang;Mingqiang Ai;F. Huang
中科院分区：
文献类型：
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作者：
Fangfang Wang;Mingqiang Ai;F. Huang
建立了一种考马斯亮蓝G-250（CBBG）与碱溶性木质素非共价结合的新方法。染料与碱溶性木质素的结合导致在630 nm或640 nm的最大波长处的可见吸收增加。最大吸收峰处的吸光度与碱溶木质素浓度呈线性关系。结果表明，新方法和酸化后重量法测定结果比紫外光谱法更接近定量信息。通过等温滴定量热实验和傅里叶变换红外光谱（FTIR）对比分析水、4%乙醇和95%乙醇洗涤后的沉淀物，表明CBBG与碱溶性木质素发生了非共价结合。这种潜在的方法是可重复的，快速的，廉价的，有很少或没有干扰碳水化合物的降解产物。
A new method involving the non-covalent binding of Coomassie Brilliant Blue G-250 (CBBG) to alkali-soluble lignin was developed. The binding of the dye to alkali-soluble lignin caused an increase in visible absorption at the maximum wavelength of 630 nm or 640 nm. A linear correlation of the absorbance at their maximum absorbing peak with alkali-soluble lignin concentration was observed. Lignin estimation in black liquor showed that the result of the new method and the gravimetric methods after acidification were closer to quantitative information than that obtained from UV spectroscopy. The isothermal titration calorimetric experiments, and Fourier Transform Infrared (FTIR) spectroscopy comparative analysis of precipitates washed by water, 4% ethanol, and 95% ethanol indicated that CBBG was bound to alkali-soluble lignin, and the binding was non-covalent. This potential method is reproducible, rapid, and cheap, and there is little or no inference from carbohydrate degradation products.