Ion transfer and adsorption behavior of ionizable drugs affected by PAMAM dendrimers at the water|1,2-dichloroethane interface
Ion transfer and adsorption behavior of ionizable drugs affected by PAMAM dendrimers at the water|1,2-dichloroethane interface
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PAMAM树枝状大分子影响可电离药物在水|1,2-二氯乙烷界面的离子转移和吸附行为
DOI：
10.1016/j.electacta.2016.01.122
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发表时间：
2016
影响因子：
6.6
通讯作者：
Hisanori Imura
中科院分区：
文献类型：
--
作者：
Hiroki Sakae;Hirohisa Nagatani;Hisanori Imura
可电离药物分子，即双嘧达莫（DIP）、普萘洛尔（PRO）和华法林（WAR）在水中的转移和吸附反应|研究了在羧酸酯封端的第3.5代（G3.5）或氨基封端的第4代（G4）聚酰胺胺（PAMAM）树枝状聚合物存在下1，2-二氯乙烷（DCE）界面。通过伏安法分析离子转移响应，确定了可电离药物的离子分配图。在DIP体系中，在正电位区观察到与界面吸附相关的附加伏安响应。虽然在水溶液中的药物种类的光谱特征几乎没有受到影响的树枝状聚合物，在DIP和PRO系统中的离子转移电流降低在G3.5 PAMAM树枝状聚合物的存在下，表明阳离子药物和带负电荷的树枝状聚合物在界面区域之间的分子间缔合。通过电位调制荧光（PMF）光谱详细研究了荧光DIP物种的界面机制。PMF结果表明，单质子化形式HDIP+通过水转移|DCE界面伴随着吸附过程。树枝状聚合物显着改变了DIP物质的界面机制，具体取决于pH条件。在酸性条件下，带正电荷的G3.5 PAMAM树枝状大分子吸附在界面上有效地阻止了HDIP+的共吸附。在较高的pH值下，DIP（或HDIP+）与树枝状聚合物的疏水内部部分（或带负电荷的外围）相互作用。
The transfer and adsorption reactions of ionizable drug molecules, i.e. dipyridamole (DIP), propranolol (PRO) and warfarin (WAR), at the water|1,2-dichloroehtane (DCE) interface were studied in the presence of the carboxylate- terminated generation 3.5 (G3.5) or amino-terminated generation 4 (G4) polyamidoamine (PAMAM) dendrimers. The ionic partition diagram of the ionizable drugs was determined through the voltammetric analysis of ion transfer responses. In the DIP system, the additional voltammetric responses associated with the interfacial adsorption were observed in the positive potential region. Although the spectroscopic features of the drug species in the aqueous solution were hardly affected by the addition of the dendrimers, the ion transfer currents in the DIP and PRO systems were decreased in the presence of the G3.5 PAMAM dendrimer indicating the intermolecular association between the cationic drugs and negatively charged dendrimers in the interfacial region. The interfacial mechanism of the fluorescent DIP species was investigated in detail by potential-modulated fluorescence (PMF) spectroscopy. The PMF results demonstrated that the monoprotonated form, HDIP+, was transferred across the water|DCE interface accompanied by the adsorption process. The interfacial mechanism of the DIP species was significantly modified by the dendrimer, depending on the pH condition. Under acidic conditions, the positively charged G3.5 PAMAM dendrimer adsorbed at the interface effectively prevented the coadsorption of HDIP+. At higher pHs, DIP (or HDIP+) interacted with the hydrophobic interior moiety (or negatively charged periphery) of the dendrimers.