Participation of nitric oxide reductase in survival of Pseudomonas aeruginosa in LPS-activated macrophages
Participation of nitric oxide reductase in survival of Pseudomonas aeruginosa in LPS-activated macrophages
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DOI：
10.1016/j.bbrc.2007.02.017
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发表时间：
2007-04-06
影响因子：
3.1
通讯作者：
Fukumori, Yoshihiro
中科院分区：
文献类型：
--
作者：
Kakishima, Kohei;Shiratsuchi, Akiko;Fukumori, Yoshihiro
一氧化氮（NO）在宿主防御系统的抗菌活性中起着至关重要的作用。我们研究了铜绿假单胞菌NO还原酶在吞噬细胞中作为解毒酶的功能。我们发现，生长的铜绿假单胞菌的NO还原酶缺陷型突变体在微氧条件下被抑制的外源性NO。此外，细胞内的生存测定NO生产RAW 264.7巨噬细胞内显示，野生型菌株存活时间长于NO还原酶缺陷型突变体。这些结果表明，铜绿假单胞菌NO还原酶可能有助于细胞内的生存作为一个反组分对宿主的防御系统。(c)2007年爱思唯尔公司All rights reserved.
Nitric oxide (NO) plays a crucial role in the antimicrobial activity of host defense systems. We investigated the function of Pseudomonas aeruginosa NO reductase as a detoxifying enzyme in phagocytes. We found that the growth of the NO reductase-deficient mutant of P. aeruginosa under a microaerobic condition was inhibited by the exogenous NO. Furthermore, the intracellular survival assay within the NO-producing RAW 264.7 macrophages revealed that the wild-type strain survived longer than the NO reductase-deficient mutant. These results suggest that the P. aeruginosa NO reductase may contribute to the intracellular survival by acting as a counter component against the host's defense systems. (c) 2007 Elsevier Inc. All rights reserved.