DIFFERENTIAL STEROID-PRODUCTION BETWEEN THECA INTERNA AND THECA EXTERNA CELLS - A 3-CELL MODEL FOR FOLLICULAR STEROIDOGENESIS IN AVIAN SPECIES
DIFFERENTIAL STEROID-PRODUCTION BETWEEN THECA INTERNA AND THECA EXTERNA CELLS - A 3-CELL MODEL FOR FOLLICULAR STEROIDOGENESIS IN AVIAN SPECIES
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DOI：
10.1210/endo-125-1-109
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发表时间：
1989-07-01
期刊：
影响因子：
4.8
通讯作者：
ELHALAWANI, ME
中科院分区：
文献类型：
--
作者：
PORTER, TE;HARGIS, BM;ELHALAWANI, ME
在鸟类中，颗粒细胞产生黄体酮(P)，但不产生睾酮(T)或雌二醇(E)。鸟类的卵泡产生T和E，解剖上分为内卵泡和外卵泡。然而，目前尚不清楚T和E是否由同一种细胞类型产生。本研究将成年雌性火鸡的排卵前卵泡分为颗粒、内膜和外膜三个解剖层，并对各层的相对类固醇作用进行了研究。排卵前最大（F1）和第五大（F5）卵泡颗粒细胞对LH （oLH, 0-10 . μ g/ml）产生的P水平升高；然而，T和E没有检测到。F5卵泡内膜细胞和外膜细胞分别单独或联合培养。膜内细胞产生P和T，但不产生E。膜外细胞产生P、T和E。通过两层膜细胞共孵育，E的产生大大增强。oLH （0-100 ng/ml）刺激卵泡内膜和卵泡外膜细胞共孵育产生P、T和E。对来自F1和F5卵泡的内膜和外膜细胞进行检测，F5卵泡外膜细胞能将雄烯二酮和T转化为E，而F1卵泡外膜细胞不能将P转化为E，这表明随着卵泡成熟，外膜的芳香酶活性下降。F1和F5膜内细胞都不能将P、A或T转化为雌二醇，这进一步证明芳香化酶活性仅限于膜外细胞。添加oLH （0-100 ng/ml）并没有增加F5外膜细胞将T转化为E，这表明外膜产生E是由底物驱动的，不受LH直接作用的影响。禽卵巢甾体生成采用三细胞模型，其中P、T、E的主要来源分别为颗粒细胞、内膜细胞和外膜细胞。
In avian species, the granulosa cells produce progesterone (P), but not testosterone (T) or estradiol (E). The theca folliculi in avian species produces T and E and is anatomically comprised of the theca interna and the theca externa. It is not known, however, whether both T and E are produced by the same cell type. In the present study, preovulatory follicles of adult female turkeys were separated into their anatomical subdivisions, the granulosa, theca interna, and theca externa, and the relative steroidogenic contribution by each layer was investigated. Granulosa cells from the largest (F1) and fifth largest (F5) preovulatory follicles produced increasing levels of P in response to ovine LH (oLH; 0-10 .mu.g/ml); however, T and E were not detectable. Theca interna and theca externa cells from F5 follicles were cultured alone or in combination. Theca interna cells produced P and T, but not E. Theca externa cells produced P, T, and E. E production was greatly enhanced by coincubation of cells from the two theca layers. oLH, (0-100 ng/ml) stimulated theca interna P and T production and E production by the coincubation of theca interna and theca externa cells. When theca interna and theca externa cells from the F1 and F5 follicles were tested, theca externa cells from F5, but not F1, converted androstenedione and T, but not P into E, indicating a loss of aromatase activity in the theca externa with follicular maturation. Neither F1 nor F5 theca interna cells converted P, A, or T into estradiol, providing additional evidence that aromatase activity is limited exclusively to the theca externa. Addition of oLH (0-100 ng/ml) did not increase conversion of T into E by the F5 theca externa cells, suggesting that production of E by the theca externa is substrate driven and not influenced by direct LH action. Avian ovarian steroidogenesis is described using a three-cell model, with the principle sources of P, T, and E being the granulosa, theca interna, and theca externa cells, respectively.