Cell adhesion molecules and the migration of LHRH neurons during development.
Cell adhesion molecules and the migration of LHRH neurons during development.
复制标题
发育过程中细胞粘附分子和 LHRH 神经元的迁移。
DOI：
10.1006/dbio.1993.1314
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发表时间：
1993
影响因子：
2.7
通讯作者：
Brackenbury,R
中科院分区：
文献类型：
--
作者：
NorgrenJr,RB;Brackenbury,R
在胚胎发育过程中，LHRH神经元产生于嗅觉上皮，沿嗅觉神经迁移，进入前脑。我们研究了几种细胞粘附分子（CAMs）在发育中的鸡嗅觉系统和大脑中的分布，以确定这些粘附分子的差异分布是否可能在LHRH神经元迁移的途径选择中起重要作用。单标记和双标记免疫细胞化学研究表明，高水平的N-CAM和N-cadherin在整个嗅上皮中表达，而不局限于大多数LHRH神经元起源的嗅上皮的内半部。此外，高水平的N-CAM、Ng-CAM和n -钙粘蛋白在整个嗅觉神经中均匀表达，而迁移的LHRH神经元则局限于神经的内半部。然而，一旦LHRH神经元到达大脑，它们沿着富含N-CAM和Ng-CAM的区域向前脑内侧表面切向背侧和尾侧迁移。在大脑内迁移的第一阶段之后，LHRH神经元横向迁移。在此阶段，N-CAM和Ng-CAM免疫染色的强度与LHRH神经元的位置没有相关性。这些结果表明，N-CAM、Ng-CAM和n -钙粘蛋白在LHRH神经元通过嗅上皮和嗅神经迁移过程中不起指导作用，但迁移的LHRH神经元可能沿着N-CAM和Ng-CAM沿前脑内侧表面的“CAM-trail”迁移。
During embryogenesis, LHRH neurons arise in the olfactory epithelium, migrate along the olfactory nerve, and enter the forebrain. We have examined the distribution of several cell adhesion molecules (CAMs) in the developing chick olfactory system and brain to determine whether differential distributions of these adhesion molecules might be important in pathway choices made by migrating LHRH neurons. Single- and double-label immunocytochemical studies indicated that high levels of N-CAM and N-cadherin were expressed throughout the olfactory epithelium and not restricted to the medial half of the olfactory epithelium where most of the LHRH neurons originate. Further, high levels of N-CAM, Ng-CAM, and N-cadherin were uniformly expressed throughout the entire olfactory nerve while migrating LHRH neurons were confined to the medial half of the nerve. However, once LHRH neurons reach the brain, they migrate dorsally and caudally, tangential to the medial surface of the forebrain, along a region enriched in N-CAM and Ng-CAM. After this first stage of migration within the brain, LHRH neurons migrate laterally. At this stage, there is no correlation between the intensity of N-CAM and Ng-CAM immunostaining and the location of LHRH neurons. These results suggest that N-CAM, Ng-CAM, and N-cadherin do not play a guiding role in LHRH neuronal migration through the olfactory epithelium and olfactory nerve but that migrating LHRH neurons may follow a "CAM-trail" of N-CAM and Ng-CAM along the medial surface of the forebrain.