CAMP-dependent growth cone guidance by netrin-1
CAMP-dependent growth cone guidance by netrin-1
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DOI：
10.1016/s0896-6273(00)80414-6
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发表时间：
1997-12-01
期刊：
影响因子：
16.2
通讯作者：
Poo, MM
中科院分区：
文献类型：
--
作者：
Ming, GL;Song, HJ;Poo, MM
已知Netrin-1作为几类发育中轴突的化学引诱剂和作为其他类轴突的化学排斥剂起作用，这显然取决于由应答细胞表达的受体类型。在培养中，非洲爪蟾胚胎脊髓神经元的生长锥表现出化学吸引转向netrin-1的来源，但在cAMP的竞争性类似物或蛋白激酶A的抑制剂的存在下，表现出化学排斥反应。通过消耗细胞外钙和通过添加针对结直肠癌中netrin-1受体的阻断抗体，消除了吸引和排斥反应。因此，神经生长锥可能会对相同的引导线索做出相反的转向行为，这取决于设定胞质cAMP水平的其他一致信号。
Netrin-1 is known to function as a chemoattractant for several classes of developing axons and as a chemorepellent for other classes of axons, apparently dependent on the receptor type expressed by responsive cells. In culture, growth cones of embryonic Xenopus spinal neurons exhibited chemoattractive turning toward the source of netrin-1 but showed chemorepulsive responses in the presence of a competitive analog of cAMP or an inhibitor of protein kinase A. Both attractive and repulsive responses were abolished by depleting extracellular calcium and by adding a blocking antibody against the netrin-1 receptor Deleted in Colorectal Cancer. Thus, nerve growth cones may respond to the same guidance cue with opposite turning behavior, dependent on other coincident signals that set the level of cytosolic cAMP.