Fabrication and application of zwitterionic phosphorylcholine functionalized monoliths with different hydrophilic crosslinkers in hydrophilic interaction chromatography
Fabrication and application of zwitterionic phosphorylcholine functionalized monoliths with different hydrophilic crosslinkers in hydrophilic interaction chromatography
复制标题
不同亲水交联剂的两性离子磷酰胆碱功能化整体材料的制备及其在亲水相互作用色谱中的应用
DOI：
10.1016/j.aca.2019.12.016
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发表时间：
2020
影响因子：
6.2
通讯作者：
Jiang Zhengjin
中科院分区：
文献类型：
--
作者：
Wang Qiqin;Zhang Qiaoxuan;Huang Hao;Zhao Pengwei;Sun Lingjue;Peng Kun;Liu Xiao;Ruan Meng;Shao Huikai;Crommen Jacques;Yu Pei;Jiang Zhengjin
以2-甲基丙烯酰氧乙基磷酰胆碱（MPC）为原料，分别与1，4-双丙烯酰哌嗪（PDA）、N，N′-亚甲基双丙烯酰胺（MBA）和2-（甲基丙烯酰氧）乙基-[N-（2-甲基丙烯酰氧）乙基]磷酰胆碱（MMPC）等亲水性交联剂热引发共聚，制备了一系列两性离子磷酰胆碱功能化整体柱。系统地比较了这些MPC功能化整体柱的物理化学和色谱性能，包括柱形态、孔径分布、渗透性、柱效、保留机理和色谱电位分析。此外，评价了交联剂对这些MPC官能化整料的色谱性能的影响。色谱结果表明，MPC功能化整体柱的极性可能与交联剂的极性有关，这进一步影响柱的选择性和效率。在最佳线速度下，使用硫脲作为测试分析物，在新型聚（MPC-co-MMPC）整体柱上获得特别高的柱效率（88，000板/m）。与poly（MPC-co-MBA）和poly（MPC-co-PDA）整体柱相比，poly（MPC-co-MMPC）整体柱对极性分析物（包括碱基、核苷和苯甲酸衍生物）具有更高的分离选择性。此外，24 N-糖肽可以检测后富集与聚（MPC-co-MMPC）与19和10 N-糖肽与聚（MPC-co-MBA）和聚（MPC-co-PDA）的整体，和没有N-糖肽富集。因此，MMPC作为一种新的和替代的亲水性交联剂的两性离子聚合物整料的发展具有很大的潜力。
In this study, a series of zwitterionic phosphorylcholine functionalized monolithic columns were fabricated via the thermally initiated co-polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) and different hydrophilic crosslinkers, including 1,4-bis(acryloyl)piperazine (PDA), N,N′-methylenebisacrylamide (MBA) and 2-(methacryloyloxy)ethyl-[N-(2-methacryloyloxy)ethyl]phosphorylcholine (MMPC). The physicochemical and chromatographic properties of these MPC functionalized monoliths, including column morphology, pore size distribution, permeability, column efficiency, retention mechanism and ζ-potential analysis, were systematically compared. Furthermore, the influence of the crosslinker on the chromatographic performance of these MPC functionalized monoliths was evaluated. The chromatographic results indicate that the polarity of MPC functionalized monoliths may be related to the polarity of the crosslinker, which further affects the column selectivity and efficiency. A particularly high column efficiency (88,000 plates/m) was obtained on the novel poly(MPC-co-MMPC) monolith at optimum linear velocity using thiourea as test analyte. Compared to the poly(MPC-co-MBA) and poly(MPC-co-PDA) monoliths, the poly(MPC-co-MMPC) monolith exhibited higher separation selectivity for polar analytes, including nucleobases, nucleosides and benzoic acid derivatives. Moreover, 24N-glycopeptides could be detected after enrichment with the poly(MPC-co-MMPC) versus 19 and 10N-glycopeptides with the poly(MPC-co-MBA) and poly(MPC-co-PDA) monoliths, and noN-glycopeptide without enrichment. Therefore, MMPC has a great potential as a new and alternative hydrophilic crosslinker for the development of zwitterionic polymeric monoliths.