Evaluating Promoter Sequences for Trapping an Enhancer Activity in the Silkworm Bombyx mori
Evaluating Promoter Sequences for Trapping an Enhancer Activity in the Silkworm Bombyx mori
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DOI：
10.11416/jibs.75.89
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发表时间：
2006-06
影响因子：
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通讯作者：
K. Uchino;M. Imamura;H. Sezutsu;I. Kobayashi;Katsura Kojima;T. Kanda;T. Tamura
中科院分区：
文献类型：
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作者：
K. Uchino;M. Imamura;H. Sezutsu;I. Kobayashi;Katsura Kojima;T. Kanda;T. Tamura
为了研究启动子序列在家蚕增强子活性检测中的作用，我们构建了6个不同的GAL 4载体，分别在不同的最小启动子控制下。此外，构建了纯合UASGFP菌株并用于测试GAL4基因的表达。比较了不同GAL 4基因插入物的转基因株系中的绿色荧光蛋白（GFP）表达。当使用BmA3启动子时，观察到不同菌株中表达模式的最显著差异。因此，利用GAL4基因和BmA3启动子是检测家蚕中插入基因位置效应的最有效方法。
To investigate the effect of a promoter sequence for detecting enhancer activity in the silkworm Bombyx mori , we constructed six different GAL4 vectors under the control of different minimum promoters. In addition, a homozygous UASGFP strain was constructed and used to test expression of the GAL4 gene. Green fluorescent pro- tein (GFP) expression was compared in the transgenic strains with the different GAL4 gene inserts. The most prominent difference in the expression pattern in the different strains was observed when the Bombyx cytoplas- mic actin gene ( BmA3 ) promoter was used. We concluded that using the GAL4 gene with the BmA3 promoter would be the most useful way for detecting the positional effect of genes inserted in the silkworm.