Adults 65 Years Old and Older Have Reduced Numbers of Functional Memory T Cells to Respiratory Syncytial Virus Fusion Protein
Adults 65 Years Old and Older Have Reduced Numbers of Functional Memory T Cells to Respiratory Syncytial Virus Fusion Protein
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DOI：
10.1128/cvi.00580-12
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发表时间：
2013-02-01
影响因子：
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通讯作者：
Tang, Roderick S.
中科院分区：
文献类型：
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作者：
Cherukuri, Anu;Patton, Kathryn;Tang, Roderick S.
呼吸道合胞病毒（RSV）感染老年人（>= 65岁），引起医疗护理的疾病和住院。虽然RSV中和抗体水平与老年人中RSV相关的住院治疗呈负相关，但RSV特异性T细胞在预防老年人疾病中的作用仍不清楚。我们检测了健康老年人（65 - 85岁）和年轻人（20 - 30岁）的RSV特异性体液、粘膜和细胞免疫谱。老年人（10.5 +/- 2.2 log（2））和年轻人（10.5 +/- 2.1 log（2））的RSV中和抗体滴度相似。相比之下，老年人（180 +/- 80个斑点形成细胞[SFC]/10（6）个外周血单核细胞[PBMC]）的RSV F蛋白特异性γ干扰素（IFN-γ）产生T细胞水平低于年轻人（1，250 +/- 420个SFC/10（6）个PBMC）。与年轻人相比，老年人PBMC培养上清液中白细胞介素-13（IL-13; 3，000 +/-1，000 pg/ml）水平较高，RSV F特异性CD 107 a（+）CD 8（+）T细胞频率较低（3.0% +/- 1.6% vs 5.0% +/- 1.6%）。这些结果表明，RSV F特异性T细胞应答缺陷导致老年人对严重RSV疾病的易感性。
Respiratory syncytial virus (RSV) infects elderly (>= 65 years) adults, causing medically attended illness and hospitalizations. While RSV neutralizing antibody levels correlate inversely with RSV-associated hospitalization in the elderly, the role of RSV-specific T cells in preventing disease in the elderly remains unclear. We examined RSV-specific humoral, mucosal, and cellular immune profiles in healthy elderly (65 to 85 years) and young (20 to 30 years) adults. RSV neutralization antibody titers in the elderly (10.5 +/- 2.2 log(2)) and young (10.5 +/- 2.1 log(2)) were similar. In contrast, levels of RSV F protein-specific gamma interferon (IFN-gamma)-producing T cells were lower in elderly (180 +/- 80 spot-forming cells [SFC]/10(6) peripheral blood mononuclear cells [PBMC]) than in young adults (1,250 +/- 420 SFC/10(6) PBMC). Higher levels of interleukin-13 (IL-13; 3,000 +/- 1,000 pg/ml) in cultured PBMC supernatants and lower frequency of RSV F-specific CD107a(+) CD8(+) T cells (3.0% +/- 1.6% versus 5.0% +/- 1.6%) were measured in PBMC from elderly than young adults. These results suggest that deficient RSV F-specific T cell responses contribute to susceptibility to severe RSV disease in elderly adults.