THE INFLUENCE OF IRRADIANCE ON THE BIOCHEMICAL-COMPOSITION OF THE PRYMNESIOPHYTE ISOCHRYSIS SP (CLONE T-ISO)
THE INFLUENCE OF IRRADIANCE ON THE BIOCHEMICAL-COMPOSITION OF THE PRYMNESIOPHYTE ISOCHRYSIS SP (CLONE T-ISO)
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DOI：
10.1111/j.0022-3646.1993.00601.x
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发表时间：
1993-10-01
影响因子：
2.9
通讯作者：
LEROI, JM
中科院分区：
文献类型：
--
作者：
BROWN, MR;DUNSTAN, GA;LEROI, JM
研究了不同光照强度对海水养殖常用的等鞭金藻（Isochrysis sp.，Parke; clone T-ISO）生化组成的影响。培养物在12：12 h光：暗（L：D）方案下生长，5个辐照度范围为50 - 1000 μ E.m（-2）.s（-1），并在对数后期收获用于生化组成分析。总成分在辐照度范围内变化。在100和250 μ E.m（-2）.s（-1）条件下培养的细胞中，蛋白质水平最高，碳水化合物和脂质水平最低的条件是50 μ E.m（-2）.s（-1）。由于细胞干重在较低辐照度下降低，当结果表示为干重百分比时，不同的趋势是明显的。蛋白质百分比在50和100 μ E.m（-2）.s（-1）时最高，碳水化合物在100 μ E.m（-2）.s（-1）时最高。谷氨酸和天冬氨酸始终以高比例（9.0-13.5%）存在，组氨酸、蛋氨酸、色氨酸、胱氨酸和羟脯氨酸是次要成分（0.0-2.6%）。葡萄糖是所有培养物中的主要糖，范围从总多糖的23.0%（50 μ E.m（-2）.s（-1））到45.0%（100 μ E.m（-2）.s（-1））。糖的比例与辐照度之间没有相关性，脂类成分和脂肪酸的比例随辐照度变化不大。主要脂肪酸为14：0、16：0、16：1（n-7）、18：1（n-9）、18：3（n-3）、18：4（n-3）、18：5（n-3）和22：6（n-3）。22：6（n-3）的比例随着辐照度的增加而增加，而18：3（n-3）、18：3（n-6）和18：4（n-3）的比例随着辐照度的增加而减少。在50 μ E.m（-2）.s（-1）生长的培养物中，色素浓度最高，但岩藻黄质和硅甲藻黄质除外（100 μ E.m（-2）.s（-1））。辅助色素的浓度与叶绿素a的浓度相关，叶绿素a的浓度随着光照的增加而降低。
The effect of irradiance on the biochemical composition of the prymnesiophyte microalga, Isochrysis sp. (Parke; clone T-ISO) a popular species for mariculture, were examined. Cultures were grown under a 12:12 h light:dark (L:D) regime at five irradiances ranging from 50 to 1000 mu E.m(-2).s(-1) and harvested at late-logarithmic phase for analysis of biochemical composition. Gross composition varied over the range of irradiances. The highest levels of protein were present in cells from cultures grown at 100 and 250 mu E.m(-2).s(-1), and minimum levels of carbohydrate and lipid occurred at 50 mu E.m(-2).s(-1). Because the cell dry weight was reduced at lower irradiances, different trends were evident when results were expressed as percentage of dry weights. Protein percentages were highest at 50 and 100 mu E.m(-2).s(-1) and carbohydrate at 100 mu E.m(-2).s(-1)The composition of amino acids did not differ over the range of irradiances. Glutamate and aspartate were always present in high proportions (9.0-13.5%) histidine, methionine, tryptophan, cystine, and hydroxy-proline were minor constituents (0.0-2.6%). Glucose was the predominant sugar in all cultures, ranging from 23.0% (50 mu E.m(-2).s(-1)) to 45.0% (100 mu E.m(-2).s(-1)) of total polysaccharide. No correlation was found between the proportion of any of the sugars and irradiance.The proportions of the lipid class components and fatty acids showed little change with irradiance. The main fatty acids were 14:0, 16:0, 16:1(n-7), 18:1(n-9), 18:3(n-3), 18:4(n-3), 18:5(n-3), and 22:6(n-3). Proportions of 22:6(n-3) increased, whereas 18:3(n-3), 18:3(n-6), and 18:4(n-3) decreased, with increasing irradiance. Pigment concentrations were highest in cultures grown at 50 mu E.m(-2).s(-1), except for fucoxanthin and diadinoxanthin (100 mu E.m(-2).s(-1)). The concentrations of accessory pigments correlated with chlorophyll a, which decreased in concentration with increasing irradiance.