ELICITATION OF SPREADING DEPRESSION BY ROSE BENGAL PHOTODYNAMIC ACTION
ELICITATION OF SPREADING DEPRESSION BY ROSE BENGAL PHOTODYNAMIC ACTION
复制标题
孟加拉玫瑰的光动力作用引发抑郁症的传播
DOI：
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发表时间：
1989
影响因子：
3.3
通讯作者：
H. Martins
中科院分区：
文献类型：
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作者：
Marialia Netto;H. Martins
扩散性抑制是指神经组织的普通电活动的缓慢传播抑制。它可以由不同类型的物理或化学非特异性刺激引起。各种证据表明涉及细胞膜的短暂改变。因此，考虑到自由基对细胞膜的作用，已经研究了染料光活化引发的反应。在黑暗中用林格溶液灌注的离体小鸡视网膜，在接受持续 5 分钟的 1 µ,M 玫瑰红脉冲，然后接受 2.1×104 至 4.2 倍 104 Jm-2 光脉冲时，能够定期表现出扩散性抑制。通过目视检查伴随反应的光散射乳波或通过记录其特有的缓慢电压变化来监测这种现象。如果注入染料的视网膜 (a) 保持在黑暗中，则不会触发反应； (b) 用红光照射（3.75 倍 102 至 2.25 倍 104 Jm-2），或 (c) 用白光刺激，但注入氮饱和溶液。结论是，在目前条件下，扩散性抑郁症的引发取决于氧气存在下孟加拉玫瑰的光活化。
Spreading depression refers to a slowly propagating depression of the ordinary electrical activity of the nervous tissue. It can be elicited by different types of physical or chemical non‐specific stimuli. Various evidences suggest that transient alterations of cell membranes are involved. For this reason, and considering the action of free radicals on cell membranes, the elicitation of the reaction by dye photoactivation has been investigated. Isolated chick retina superfused in the dark with Ringer solution was able to regularly exhibit spreading depression when submitted to 1 µ,M rose bengal pulse of 5 min in duration, followed by 2.1 104 to 4.2 times 104 Jm‐2 light pulse. The phenomenon was monitored either by visual inspection of the light‐scattering milky wave that accompanies the reaction or by recording its characteristic slow voltage variation. The reaction was not triggered if the retina, superfused with the dye, was (a) maintained in the dark; (b) illuminated with red light (3.75 times 102 to 2.25 times 104 Jm‐2), or (c) stimulated by white light but superfused with nitrogen‐saturated solutions. It is concluded that, under the present conditions, the elicitation of spreading depression is contingent on the photoactivation of rose bengal in the presence of oxygen.