Restoration of intact anthers in a thermosensitive, antherless, male-sterile cultivar of Asiatic hybrid lily in response to high temperature
Restoration of intact anthers in a thermosensitive, antherless, male-sterile cultivar of Asiatic hybrid lily in response to high temperature
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亚洲杂交百合热敏无花药雄性不育品种完整花药对高温的恢复
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
K. Miyoshi
中科院分区：
文献类型：
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作者：
T. Sato;K. Miyoshi
摘要 亚洲杂交百合品种“秋田小白”（APW）是雄性不育，雄蕊无花药。这一特性对于花匠来说是有利的，否则他们必须在花朵绽放后小心地去除花药。然而，出乎意料的是，我们在园艺环境中对该品种进行田间试验时，在 400 株植物中不到 1% 的几朵花中观察到一两个具有完整花药的雄蕊。该试验使用在 –2°C 下储存 9 个月的灯泡进行。本研究的目的是确定调节 APW 百合无花药表型表达和逆转的因素。我们用冷冻储存的球茎在生长柜的三种温度条件下种植 APW 植物。温度条件为：高温 32°C/25°C（白天/夜间）、中度 25°C/18°C 或低温 18°C/11°C；光周期均为14小时。高温条件下生长的植株所有雄蕊均具有完整的花药。在中等温度条件下，所有受检植物的所有花丝边缘均呈淡黄色且肿胀，类似于未发育的花药。低温条件下，无花药表型稳定，没有雄蕊完整或未发育的花药。来自高温条件下生长的植物的完整花药的花粉在体外发芽并以正常方式产生营养和生殖核。用这些花粉粒授粉后产生的有活力种子的产量几乎与其他亚洲杂交百合的产量相同（通过各种杂交组合，每个胶囊获得 96 – 113 粒种子）。讨论了温度对无花药表型的调节。
Summary The Asiatic hybrid lily cultivar ‘Akita Petit White’ (APW) is male-sterile with antherless stamens. This trait is advantageous for florists, who otherwise have to remove anthers carefully after the flowers have bloomed. However, unexpectedly, we observed one or two stamens with intact anthers in a few flowers, on fewer than 1% of a total of 400 plants in a field trial of this cultivar, in a horticultural setting. The trial was performed with bulbs that had been stored at –2°C for 9 months. The purpose of the present study was to identify those factors that regulate the expression and reversal of the antherless phenotype in APW lilies. We grew APW plants, from bulbs that had been stored frozen, under three temperature regimes in growth cabinets. Temperature conditions were: high 32°C/25°C (day/night), moderate 25°C/18°C, or low 18°C/11°C; all with a 14 h photoperiod.All stamens in plants which had been grown under the high temperature conditions had intact anthers. Under the moderate temperature conditions, the edges of all filaments, in all plants examined, were yellowish and swollen, resembling undeveloped anthers. Under the low temperature conditions, the antherless phenotype was stable, and no stamens had intact or undeveloped anthers. Pollen from intact anthers, from plants grown under the high temperature conditions, germinated in vitro and produced vegetative and generative nuclei in the normal manner. The yields of viable seeds produced after pollination with these pollen grains were almost equal to those of other Asiatic hybrid lilies (96 – 113 seeds per capsule were obtained with various hybrid combinations). Regulation of the antherless phenotype by temperature is discussed.