A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES
A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES
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DOI：
10.1111/j.1399-3054.1962.tb08052.x
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发表时间：
1962-01-01
影响因子：
6.4
通讯作者：
SKOOG, F
中科院分区：
文献类型：
--
作者：
MURASHIGE, T;SKOOG, F
将烟草叶提取物加入到标准改良的怀特营养培养基中，可使威斯康星州38号烟草的离体髓或愈伤组织培养物的产量增加几倍。在某种程度上，这种增加是由于提取物的无机成分，特别是N和K，这可以由提取物的灰分或介质中N和K盐的水平升高来代替。这种增加部分是由于提取物的有机成分增加了培养物的生长速率和最终鲜重。在改良的培养基中加入赤霉酸和Braun's嘌呤和氨基酸补充物，生长速率也有类似的增加，但最终产量没有增加。已开发出一种修订的培养基，其中提供了足够量的每种元素，以确保引入植物组织提取物等中通常预期范围内的额外量不会导致产量增加。除蔗糖外，有机成分保持不变已提高至3%，加入肌醇作为常规成分，加入Edamin作为任选成分。改良培养基设计用于有机生长因子的生物测定。它提供了快速的生长速度，增加对有机生长因子的反应，并将无机和常见有机营养素的干扰降至最低。在植物提取物的存在下，在50 ml培养基上获得了高达35 g的鲜重产量。在这些条件下，水似乎是所用烟草组织生长的限制因素。植物组织培养的一般应用，限制和行为方面进行了讨论。
A several-fold increase in yield of excised pith or callus cultures of Nicotiana tabacum, variety Wisconsin 38 was obtained by addition of leaf extract to the standard modified White''s nutrient medium. In part the increase was due to inorganic constituents of the extract, especially N and K, which could be substituted for by either the ash of the extract or raised levels of N and K salts in the medium. In part the increase was due to organic constituents of the extract which increased both the growth rate and final fresh weight of the cultures. Similar increases in growth rate but not in final yield were obtained by adding gibberellic acid and Braun''s supplements of purines and amino acids to a revised medium. A revised medium has been developed with each element provided in sufficient quantity to insure that no increase in yield will result from the introduction of additional amounts in the range ordinarily to be expected in plant tissue extracts, etc. The organic constituents have been retained unchanged except that sucrose has been raised to 3%, myo-inositol has been added as a regular constituent and Edamin has been introduced as an optional constituent. The revised medium is designed for use in bioassays of organic growth factors. It provides for rapid growth rate, increased response to organic growth factors and minimal interference from inorganic and common organic nutrients. In the presence of plant extracts fresh weight yields of up to 35 g on 50 ml of medium have been obtained. Under these conditions water appears to be the limiting factor for growth of the tobacco tissues employed. Aspects of general application, limitations and behavior of plant tissue cultures are discussed.