Essential role of the small GTPase Rac in disease resistance of rice.
Essential role of the small GTPase Rac in disease resistance of rice.
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DOI：
10.1073/pnas.98.2.759
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发表时间：
2001-01
影响因子：
11.1
通讯作者：
Eiichiro Ono;H. Wong;T. Kawasaki;M. Hasegawa;O. Kodama;K. Shimamoto
中科院分区：
文献类型：
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作者：
Eiichiro Ono;H. Wong;T. Kawasaki;M. Hasegawa;O. Kodama;K. Shimamoto
活性氧中间产物(ROI)的产生和一种称为过敏性反应(HR)的程序性细胞死亡通常与植物的抗病能力有关。我们之前已经证明，水稻的RAC同源物Osrac1是水稻ROI产生和细胞死亡的调节因子。在这里，我们证明了具有结构性活性的Osrac1(I)引起类似HR的反应，并极大地减少了对稻瘟病菌的致病小种的病害；(Ii)引起对白叶枯病的致病小种的抗性；以及(Iii)引起植保素的产生，并改变了防御相关基因的表达。在转基因细胞培养中，显性负性Osrac1抑制激发子诱导的ROI的产生，在植物中抑制无毒小种诱导的HR。综上所述，我们的发现有力地表明，Osrac1在水稻抗病中具有普遍的作用。
Production of reactive oxygen intermediates (ROI) and a form of programmed cell death called hypersensitive response (HR) are often associated with disease resistance of plants. We have previously shown that the Rac homolog of rice, OsRac1, is a regulator of ROI production and cell death in rice. Here we show that the constitutively active OsRac1 (i) causes HR-like responses and greatly reduces disease lesions against a virulent race of the rice blast fungus; (ii) causes resistance against a virulent race of bacterial blight; and (iii) causes enhanced production of a phytoalexin and alters expression of defense-related genes. The dominant-negative OsRac1 suppresses elicitor-induced ROI production in transgenic cell cultures, and in plants suppresses the HR induced by the avirulent race of the fungus. Taken together, our findings strongly suggest that OsRac1 has a general role in disease resistance of rice.