Posttranslational modification and microtubule stability.
Posttranslational modification and microtubule stability.
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DOI：
10.1083/jcb.105.5.2167
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发表时间：
1987-11
期刊：
影响因子：
--
通讯作者：
Kirschner M
中科院分区：
文献类型：
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作者：
Schulze E;Asai DJ;Bulinski JC;Kirschner M
我们已经探讨了微管蛋白翻译后修饰和微管稳定性之间的关系，使用抗体阻断技术的变化。在人视网膜母细胞瘤细胞中，我们发现乙酰化和去酪化微管代表了细胞总微管的一致亚群。我们还发现，注射生物素-微管蛋白后1小时内未发生聚合的稳定微管均被翻译后修饰；此外，动态微管均未被修饰。因此，我们得出结论，在这些细胞中，稳定的、乙酰化的和去甲基化的微管代表了细胞总网络的相同子集。然而，翻译后修饰并不是微管稳定性的先决条件，反之亦然。胚胎三趾动物肾细胞没有检测到乙酰化微管，但有相当数量的稳定微管，鸡胚胎成纤维细胞被广泛修饰，但稳定微管很少。我们得出结论，不同的细胞类型可以通过调节翻译后修饰和微管周转的相对速率来产生特定的微管亚群。
We have probed the relationship between tubulin posttranslational modification and microtubule stability, using a variation of the antibody-blocking technique. In human retinoblastoma cells we find that acetylated and detyrosinated microtubules represent congruent subsets of the cells' total microtubules. We also find that stable microtubules defined as those that had not undergone polymerization within 1 h after injection of biotin-tubulin were all posttranslationally modified; furthermore dynamic microtubules were all unmodified. We therefore conclude that in these cells the stable, acetylated, and detyrosinated microtubules represent the same subset of the cells' total network. Posttranslational modification, however, is not a prerequisite for microtubule stability and vice versa. Potorous tridactylis kidney cells have no detectable acetylated microtubules but do have a sizable subset of stable ones, and chick embryo fibroblast cells are extensively modified but have few stable microtubules. We conclude that different cell types can create specific microtubule subsets by modulating the relative rates of posttranslational modification and microtubule turnover.