Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction
Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction
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DOI：
10.1534/genetics.106.060467
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发表时间：
2006-10-01
期刊：
影响因子：
3.3
通讯作者：
Dawe, R. Kelly
中科院分区：
文献类型：
--
作者：
Luce, Amy C.;Sharma, Anupma;Dawe, R. Kelly
即使在遗传分辨率很高的物种中，着丝粒也很难绘制。在这里，我们表明，重复之间的功能提供了可靠的单拷贝市场重组自交系内着丝粒和近着丝粒异染色质映射。将重复功能作图与抗CENH 3介导的ChIP相结合，以提供玉米着丝粒8的确定性图谱位置。
Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that functions between repeats provide reliable single-copy markets for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat function mapping was combined will anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.