NUCLEOTIDE-SEQUENCE AND GENOME ORGANIZATION OF FOOT-AND-MOUTH-DISEASE VIRUS
NUCLEOTIDE-SEQUENCE AND GENOME ORGANIZATION OF FOOT-AND-MOUTH-DISEASE VIRUS
复制标题
DOI：
10.1093/nar/12.16.6587
复制
发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
SCHALLER, H
中科院分区：
文献类型：
--
作者：
FORSS, S;STREBEL, K;SCHALLER, H
从克隆的口蹄疫病毒cDNA中获得了一段7802个核苷酸的连续序列，该序列覆盖了94%的口蹄疫病毒RNA的5′ -端poly（C）区和3′ -端poly（A）区之间，总RNA基因组长度校正为8450个核苷酸。在该序列中鉴定了一个tong开放读框，该读框从RNA基因组的5端开始约1300个碱基，并从其多聚腺苷酸化的3端延伸至终止密码子92个碱基。由该编码序列推导出的2332个氨基酸的蛋白质序列与260 K FMDV多聚蛋白序列相对应。它的加工位点和12个成熟病毒蛋白质推断从蛋白质数据，可用于一些蛋白质，预测的切割特异性的FMDV编码的蛋白酶的Glu / Gly（苏氨酸，丝氨酸）连接，和同源性相关的蛋白质从脊髓灰质炎病毒。此外，通过与多蛋白起始信号的序列同源性和体外翻译研究，已经鉴定了92个密码子的短的未连接的阅读框架。
A continuous 7802 nucleotide sequence spanning the 94% of foot and mouth disease virus RNA between the 5′ -proximal poly(C) tract and the 3′ -terminal poty(A) was obtained trom cloned cDNA, and the total size of the RNA genome was corrected to 8450 nucleotides. A tong open readin frame was identified within this sequence starting about 1300 bases from the 5 end of the RNA genome and extending to a termination codon 92 bases from its polyadenylated 3 end. The protein sequence of 2332 amino acids deduced trom this coding sequence was correlated with the 260 K FMDV polyprotein. Its processing sites and twelve mature viral proteins were inferred from protein data, available for some proteins, a predicted cleavage specificity of an FMDV encoded protease for Glu / Gly(Thr, Ser) linkages, and homologies to related proteins from poliovirus. In addition, a short unlinked reading frame of 92 codons has been identified by sequence homology to the polyprotein initiation signal and by in vitro translation studies.