Botulinum neurotoxin type A alleviates mechanical hypersensitivity associated with infraorbital nerve constriction injury in rats
Botulinum neurotoxin type A alleviates mechanical hypersensitivity associated with infraorbital nerve constriction injury in rats
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DOI：
10.1016/j.neulet.2016.11.043
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发表时间：
2017-01-10
影响因子：
2.5
通讯作者：
Iwata, Koichi
中科院分区：
文献类型：
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作者：
Noma, Noboru;Watanabe, Kosuke;Iwata, Koichi
我们研究了A型肉毒杆菌神经毒素（BoNT-A）对大鼠眶下神经收缩（ION-CCI）相关的机械性异常性疼痛和痛觉过敏的影响。ION-CCI大鼠在术后第7天接受皮下BoNT-A注射到胡须垫区域，并在术后第14天和第21天进行疼痛评估。将大鼠分配到四个治疗组之一（每组n = 5）：ION-CCI+ BoNT-A 20 μ g（低剂量组）、ION-CCI+ BoNT-A 200 μ g（高剂量组）、ION-CCI+盐水和假手术组。在术前（基线）和术后第7、14和21天评价机械性异常性疼痛和痛觉过敏。在触须垫皮肤伤害性机械刺激后，分析三叉神经脊束核尾侧亚核（Vc）和上颈髓（C1-C2）内磷酸化细胞外信号调节激酶（pERK）免疫反应（IR）神经元的数量和分布。在第21天，通过高剂量而不是低剂量BoNT-A施用减弱疼痛反应行为。此外，在触须垫皮肤伤害性机械刺激后，高剂量BoNT-A组Vc和C1-C2浅层pERK-IR细胞数量显著低于ION-CCI+生理盐水组。目前的研究结果表明，通过抑制Vc神经元的活动，高剂量皮内注射的BONT-A在ION神经支配的网站adminates机械性面部异常性疼痛和痛觉过敏与ION-CCI。(C)2016爱思唯尔爱尔兰有限公司版权所有。
We investigated the effect of botulinum neurotoxin type A (BoNT-A) on mechanical allodynia and hyperalgesia associated with infraorbital nerve constriction (ION-CCI) in rats. ION-CCI rats received a subcutaneous BoNT-A injection into the whisker pad area on day 7 postoperatively and underwent pain assessment on days 14 and 21 postoperatively. Rats were assigned to one of four treatment groups (n = 5 each): ION-CCI+ BoNT-A 20 pg (low-dose group), ION-CCI+ BoNT-A 200 pg (high-dose group), ION-CCI+ saline, and Sham. Mechanical allodynia and hyperalgesia were evaluated preoperatively (baseline) and on days 7, 14, and 21 postoperatively. After noxious mechanical stimulation of whisker pad skin, the number and distribution pattern of the phosphorylated extracellular signal-regulated kinase (pERK)-immunoreactive (IR) neurons were analyzed in the trigeminal spinal subnucleus caudalis (Vc) and upper cervical spinal cord (C1-C2). On day 21, nocifensive behavior was attenuated by high-dose but not low-dose BoNT-A administration. In addition, after noxious mechanical stimulation of whisker pad skin, the numbers of pERK-IR cells in the superficial laminae of Vc and C1-C2 were significantly lower in the high dose BoNT-A group than in the ION-CCI+ saline group. The present findings suggest that, by suppressing Vc neuronal activity, high-dose intradermal injection of BoNT-A at the site of ION innervation alleviates mechanical facial allodynia and hyperalgesia associated with ION-CCI. (C) 2016 Elsevier Ireland Ltd. All rights reserved.