The first gene in the Escherichia coli secA operon, gene X, encodes a nonessential secretory protein.
The first gene in the Escherichia coli secA operon, gene X, encodes a nonessential secretory protein.
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大肠杆菌 secA 操纵子中的第一个基因 X 基因编码非必需分泌蛋白。
DOI：
10.1128/jb.173.22.7092-7097.1991
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发表时间：
1991
影响因子：
3.2
通讯作者：
Oliver,DB
中科院分区：
文献类型：
--
作者：
Rajapandi,T;Dolan,KM;Oliver,DB
在大肠杆菌secA操纵子的第一个基因，基因X，TnphoA插入分离和分析。对基因X-PhoA融合蛋白的研究表明，基因X编码分泌蛋白，因为融合蛋白具有正常的碱性磷酸酶活性，并且在周质中发现了该活性的相当大一部分。此外，最初用可切割的信号肽合成Gene X-PhoA融合蛋白。使用基因X：：TnphoA插入来构建含有基因X的破坏的染色体拷贝的菌株。对该菌株的分析表明，基因X对细胞生长和活力是非必需的，并且似乎在蛋白质输出过程中不起重要作用。
TnphoA insertions in the first gene of the Escherichia coli secA operon, gene X, were isolated and analyzed. Studies of the Gene X-PhoA fusion proteins showed that gene X encodes a secretory protein, since the fusion proteins possessed normal alkaline phosphatase activity and a substantial portion of this activity was found in the periplasm. In addition, the Gene X-PhoA fusion proteins were initially synthesized with a cleavable signal peptide. A gene X::TnphoA insertion was used to construct a strain containing a disrupted chromosomal copy of gene X. Analysis of this strain indicated that gene X is nonessential for cell growth and viability and does not appear to play an essential role in the process of protein export.