Plasma miR-124 Is a Promising Candidate Biomarker for Human Intracerebral Hemorrhage Stroke.
Plasma miR-124 Is a Promising Candidate Biomarker for Human Intracerebral Hemorrhage Stroke.
复制标题
血浆 miR-124 是人类脑出血中风的有前途的候选生物标志物
DOI：
10.1007/s12035-017-0808-8
复制
发表时间：
2018-07
影响因子：
5.1
通讯作者：
Poon WS
中科院分区：
文献类型：
--
作者：
Wang Z;Lu G;Sze J;Liu Y;Lin S;Yao H;Zhang J;Xie D;Liu Q;Kung HF;Lin MC;Poon WS
中风导致死亡或长期残疾，威胁着全世界人口的总体健康。最近的研究表明，miRNAs是失调的，可以作为生物标志物用于中风的诊断和预后。脑出血（intracerebral hemorrhage，ICH）占所有脑卒中病例的15%。然而，目前对miRNAs在ICH中的功能和临床意义知之甚少。本研究建立了胶原酶诱导的大鼠脑出血模型，以模拟人类脑出血综合征。我们分析了728个大鼠miRNAs在脑出血后不同时间点在大鼠脑组织和血浆中的表达，并鉴定了一组人脑富集的miRNAs，其在大鼠脑出血后血浆中的表达水平发生了变化。其中，miR-124的表达水平在大鼠脑出血过程中呈现出明显的同步性变化。在急性损伤期（第1、2天）明显升高，延迟恢复期（第7、14、30天）逐渐降低，恢复后期（第60天）恢复至正常水平。我们进一步确定了来自人类ICH患者的miR-124的血浆表达谱。与大鼠ICH模型中观察到的模式相似，我们的结果表明，患者到达医院后，miR-124的平均血浆浓度在24 h内增加超过100倍，然后在第2、7、14天逐渐下降，并在第30天接近正常水平。总之，这些结果强烈表明，miR-124的血浆浓度是用于人类ICH的早期检测和预测预后的有希望的候选生物标志物。
Stroke causes death or long-term disabilities and threatens the general health of the population worldwide. Recent studies have suggested that miRNAs are dysregulated and can be used as biomarkers for diagnosis and prognosis in stroke. The intracerebral hemorrhage (ICH) accounts for 15% of all the stroke cases. However, at present, little is known regarding the functions and clinical implications of miRNAs in ICH. In the present study, we established the collagenase-induced rat ICH model to mimic human ICH syndrome. We profiled the expression of 728 rat miRNAs at different time points in rat brain tissues and plasma post-ICH and identified a set human brain-enriched miRNAs that had changed expression level in the plasma of rat ICH. Among them, the expression levels of miR-124 displayed significantly synchronous alterations in rat plasma and brain tissue during ICH progression. They were significantly elevated at the acute injury phase (day 1 and 2), gradually decreased during the delayed recovery phase (day 7, 14 and 30), and finally restored to normal levels at late recovery phase (day 60). We further determined the plasma expression profile of miR-124 from human ICH patients. Similar to the pattern observed in rat ICH model, our results indicated that immediately after patients reached the hospital, the average plasma concentrations of miR-124 increased more than 100-fold in 24 h, then decreased gradually on day 2, 7, 14 and to near normal level on day 30. Taken together, these results strongly suggested that plasma concentration of miR-124 is a promising candidate biomarker for the early detection and predictive prognosis of human ICH.
登录
查看更多内容
影响因子：
8.3
作者：
ELGER, B;SEEGA, J;BRENDEL, R
通讯作者：
BRENDEL, R
影响因子：
9.9
作者：
Rosenberg, GA;Navratil, M
通讯作者：
Navratil, M
影响因子：
168.9
作者：
Qureshi, Adnan I.;Mendelow, A. David;Hanley, Daniel F.
通讯作者：
Hanley, Daniel F.
影响因子：
8.3
作者：
DeBow, SB;Davies, MLA;Colbourne, F
通讯作者：
Colbourne, F
DOI：
10.1016/j.trsl.2015.01.012
发表时间：
2015-08
期刊：
Translational research : the journal of laboratory and clinical medicine
影响因子：
--
作者：
Lenkala D;Gamazon ER;LaCroix B;Im HK;Huang RS
通讯作者：
Huang RS