Influence of the nature of the sterol on the behavior of palmitic acid/sterol mixtures and their derived liposomes
Influence of the nature of the sterol on the behavior of palmitic acid/sterol mixtures and their derived liposomes
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DOI：
10.1016/j.bbamem.2010.02.008
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发表时间：
2010-06-01
影响因子：
3.4
通讯作者：
Lafleur, Michel
中科院分区：
文献类型：
--
作者：
Cui, Zhong-Kai;Bastiat, Guillaume;Lafleur, Michel
棕榈酸（PA）和以下甾醇（二氢胆固醇，麦角甾醇，7-脱氢胆固醇，豆甾醇和豆甾烷醇），在PA/甾醇的摩尔比为3/7，形成的混合物的相行为，其特征在于在不同的pH值的IR和H-2 NMR光谱。我们的研究表明，它是可能的形成液体有序（LO）层状相与这些二元非磷脂混合物。PA在这些系统中的烷基链动力学的表征揭示了甾醇的大的有序效应。使用标准挤出技术挤出这些系统以形成大的单层囊泡（LUV）是可能的，除了含麦角甾醇的混合物的情况。由此产生的LUV显示出非常有限的被动渗透性与高甾醇浓度一致。此外，这些PA/甾醇自组装双层的稳定性也被发现是pH敏感的，因此，潜在地可用作纳米载体。通过研究不同的甾醇，我们可以建立这些双层的结构和它们的渗透性能之间的一些相关性。甾醇的C17侧链结构似乎在与脂肪酸的混合性质中起主要作用。(C)2010 Elsevier B. V.保留所有权利。
The phase behavior of mixtures formed with palmitic acid (PA) and one of the following sterols (dihydrocholesterol, ergosterol, 7-dehydrocholesterol, stigmasterol and stigmastanol), in a PA/sterol molar ratio of 3/7, has been characterized by IR and H-2 NMR spectroscopy at different pH. Our study shows that it is possible to form liquid-ordered (lo) lamellar phases with these binary non-phospholipid mixtures. The characterization of alkyl chain dynamics of PA in these systems revealed the large ordering effect of the sterols. It was possible to extrude these systems, using standard extrusion techniques, to form large unilamellar vesicles (LUVs), except in the case of ergosterol-containing mixture. The resulting LUVs displayed a very limited passive permeability consistent with the high sterol concentration. In addition, the stability of these PA/sterol self-assembled bilayers was also found to be pH-sensitive, therefore, potentially useful as nanovectors. By examining different sterols, we could establish some correlations between the structure of these bilayers and their permeability properties. The structure of the side chain at C17 of the sterol appears to play a prime role in the mixing properties with fatty acid. (C) 2010 Elsevier B.V. All rights reserved.