The methylated constituents of globin mRNA
The methylated constituents of globin mRNA
复制标题
珠蛋白 mRNA 的甲基化成分
DOI：
10.1016/0014-5793(75)80155-4
复制
发表时间：
1975
期刊：
影响因子：
3.5
通讯作者：
K. Scherrer
中科院分区：
文献类型：
--
作者：
R. Perry;K. Scherrer
Recent findings indicate that messenger RNA from a wide variety of cellular and viral sources contains methylated constituents[l-5]. Studies of complex mixtures of mammalian mRNA’s have indicated that the methyl groups occur in internal residues of N6 methyl adenylate and in unusual sequences at the 5’-termini [Sl 11. The 5’-terminal sequences appear to be of the form X5’ppp”(Nmp) 1-2N~... in which a ‘capping nucleoside’X (generally m’G), is joined by a 5’-5’triphosphate linkage to sequences containing either one or two 2’0ribose methylated nucleotides (Nmp). In order to determine whether such components are also characteristic of a well-defined species of eukaryotic mRNA, we investigated the methylated derivatives of mRNA in immature duck erythrocytes. These cells synthesize readily detectable quantities of 9 S globin mRNA in addition to a variety of other mRNA’s [121. Our findings indicate that 9 S globin mRNA also contains capped 5’-terminal sequences, but very little, if any, internal base methylated derivatives. Moreover, compared to a mixture of other mRNA species synthesized by these cells, the 9 S mRNA is relatively enriched in the sequences that contain two 2’0methyl nucleotides.
