Interfacing Microfluidics with Negative Stain Transmission Electron Microscopy.
Interfacing Microfluidics with Negative Stain Transmission Electron Microscopy.
复制标题
微流体与负染色透射电子显微镜的接口。
DOI：
10.1021/acs.analchem.5b03884
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发表时间：
2016
影响因子：
7.4
通讯作者：
Roper,MichaelG
中科院分区：
文献类型：
--
作者：
Mukhitov,Nikita;Spear,JohnM;Stagg,ScottM;Roper,MichaelG
提出了一种用于制备用于透射电子显微镜（EM）的负染色网格的微流体平台。微流控装置由蚀刻有容易制造的特征的玻璃组成，所述特征促进染色后网格的提取并保持样品的完整性。利用该装置同时减少了网格上的环境污染，并提高了重金属染色的均匀性，需要增强可视化的生物标本相比，传统制备的EM网格。这种易于使用的EM网格制备设备为具有更多集成功能的系统的未来开发提供了基础，这将允许高通量和动态结构生物学研究。
A microfluidic platform is presented for preparing negatively stained grids for use in transmission electron microscopy (EM). The microfluidic device is composed of glass etched with readily fabricated features that facilitate the extraction of the grid poststaining and maintains the integrity of the sample. Utilization of this device simultaneously reduced environmental contamination on the grids and improved the homogeneity of the heavy metal stain needed to enhance visualization of biological specimens as compared to conventionally prepared EM grids. This easy-to-use EM grid preparation device provides the basis for future developments of systems with more integrated features, which will allow for high-throughput and dynamic structural biology studies.