The Hippo signaling pathway: a potential therapeutic target is reversed by a Chinese patent drug in rats with diabetic retinopathy.
The Hippo signaling pathway: a potential therapeutic target is reversed by a Chinese patent drug in rats with diabetic retinopathy.
复制标题
Hippo信号通路：中成药逆转糖尿病视网膜病变大鼠的潜在治疗靶点
DOI：
10.1186/s12906-017-1678-3
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发表时间：
2017-04-04
影响因子：
--
通讯作者：
Han J
中科院分区：
文献类型：
--
作者：
Hao GM;Lv TT;Wu Y;Wang HL;Xing W;Wang Y;Li C;Zhang ZJ;Wang ZL;Wang W;Han J
研究背景Hippo信号通路参与了血管生成，但Hippo信号通路在糖尿病视网膜病变中的作用尚未阐明。复方血栓通胶囊已被用于治疗糖尿病视网膜病变在中国，但是，复方血栓通胶囊对海马pathway的影响还没有investigated.MethodsIn这项研究中，糖尿病引起的SD大鼠腹腔注射链脲佐菌素。20周后，给予复方血栓通胶囊治疗12周。当给药结束时，分离眼睛用于蛋白质印迹和免疫组织化学分析。结果糖尿病大鼠眼内丛状层P-MST水平降低，感光细胞层P-YAP表达减少。此外，糖尿病大鼠视网膜中的Lats、TAZ和TEAD也显著增加。结论Hippo信号通路在糖尿病视网膜病变的发生发展中起重要作用，有望成为糖尿病视网膜病变预防的靶向治疗手段。
BackgroundThe Hippo signaling pathway is reported to be involved in angiogenesis, but the roles of the Hippo pathway in diabetic retinopathy have not been addressed. Fufang Xueshuantong Capsule has been used to treat diabetic retinopathy in China; however, the effect of Fufang Xueshuantong Capsule on the Hippo pathway has not been investigated.MethodsIn this study, diabetes was induced in Sprague-Dawley rats with intraperitoneal injection of streptozotocin. Twenty weeks later, Fufang Xueshuantong Capsule was administered for 12 weeks. When the administration ended, the eyes were isolated for western blot and immunohistochemistry analyses. The levels of P- mammalian sterile 20-like (MST), large tumor suppressor homolog (Lats), P- yes-associated protein (YAP), transcriptional co-activator with PDZ binding motif (TAZ) and TEA domain family members (TEAD) were measured.ResultsDiabetic rats had a decreased P-MST level in the inner plexiform layer and reduced expression of P-YAP in the photoreceptor layers of their eyes. In addition, diabetic rats displayed remarkable increases in Lats, TAZ and TEAD in their retinas. Furthermore, Fufang Xueshuantong Capsule restored the changes in the Hippo pathway.ConclusionsThe Hippo signaling pathway is important for the progression of diabetic retinopathy and will hopefully be a targeted therapeutic approach for the prevention of diabetic retinopathy.