Molecular characterization and heterologous expression of an endo-β-1,6-glucanase gene from the mycoparasitic fungus Trichoderma harzianum
Molecular characterization and heterologous expression of an endo-β-1,6-glucanase gene from the mycoparasitic fungus Trichoderma harzianum
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真菌寄生真菌哈茨木霉内切-β-1,6-葡聚糖酶基因的分子特征和异源表达
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
José Antonio Pintor
中科院分区：
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作者：
J. Lora;J. Cruz;A. Llobell;T. Benítez;José Antonio Pintor
来自丝状真菌哈茨木霉的水解酶已被描述为木霉针对真菌植物病原体的真菌寄生作用的关键要素。在本报告中，我们描述了第一个编码 β-1,6-内切葡聚糖酶基因的基因组和 cE)NA 克隆。推导的蛋白质序列与其他β-葡聚糖酶的同源性有限。 Northern 实验显示葡萄糖对 mRNA 积累的显着抑制。通过构建 cDNA 与酵母启动子的转录融合体，该蛋白已在酿酒酵母中成功生产。该酿酒酵母重组菌株对含有 β-1,6-葡聚糖的琼脂平板显示出强烈的裂解作用。
Hydrolytic enzymes from the filamentous fungus Trichoderma harzianum have been described as critical elements of the mycoparasitic action of Trichoderma against fungal plant pathogens. In this report we describe the first genomic and cE)NA clones encoding a β-1,6-endoglucanase gene. The deduced protein sequence has limited homology with other β-glucanases. Northern experiments show a marked repression of mRNA accumulation by glucose. The protein has been successfully produced in Saccharomyces cerevisiae upon construction of a transcriptional fusion of the cDNA with a yeast promoter. This S. cerevisiae recombinant strain shows a strong lytic action on agar plates containing β-1,6-glucan.