Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes
Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes
复制标题
DOI：
10.1007/s11684-020-0792-7
复制
发表时间：
2020-09-02
影响因子：
8.1
通讯作者：
Qiao, Jie
中科院分区：
文献类型：
--
作者：
Huo, Ying;Yuan, Peng;Qiao, Jie
卵母细胞冷冻保存由于临床和社会原因而被广泛应用。以往的研究表明，传统的慢速冷冻冷冻保存程序，而不是存储时间，可以改变冷冻卵母细胞的基因表达谱。玻璃化程序和相关的冷冻储存时间是否对人类中期II卵母细胞的转录组有任何影响仍然未知。本研究招募了4名接受过IVF治疗的女性（30-32岁）。以单细胞分辨率分析3个新鲜卵母细胞和13个存活的玻璃化解冻卵母细胞（3、3、4和3个卵母细胞冷冻保存1、2、3和12个月）的RNA-Seq图谱。在13个玻璃化冷冻-解冻的卵母细胞中，共有1987个基因差异表达。然而，在1个月、2个月、3个月和12个月储存组中，任何两个组之间均未发现差异表达的基因。进一步分析表明，玻璃化冷冻卵母细胞中的异常基因与卵子发生和发育密切相关。我们的研究结果表明，玻璃化冷冻对成熟人类卵母细胞转录组的影响是由程序本身诱导的，这表明长期冷冻保存人类卵母细胞是安全的。
Oocyte cryopreservation is widely used for clinical and social reasons. Previous studies have demonstrated that conventional slow-freezing cryopreservation procedures, but not storage time, can alter the gene expression profiles of frozen oocytes. Whether vitrification procedures and the related frozen storage durations have any effects on the transcriptomes of human metaphase II oocytes remain unknown. Four women (30-32 years old) who had undergone IVF treatment were recruited for this study. RNA-Seq profiles of 3 fresh oocytes and 13 surviving vitrified-thawed oocytes (3, 3, 4, and 3 oocytes were cryostored for 1,2, 3, and 12 months) were analyzed at a single-cell resolution. A total of 1987 genes were differentially expressed in the 13 vitrified-thawed oocytes. However, no differentially expressed genes were found between any two groups among the 1-, 2-, 3-, and 12-month storage groups. Further analysis revealed that the aberrant genes in the vitrified oocytes were closely related to oogenesis and development. Our findings indicated that the effects of vitrification on the transcriptomes of mature human oocytes are induced by the procedure itself, suggesting that long-term cryostorage of human oocytes is safe.