Cell Death Induced by Subnanosecond Pulsed Electric Fields at Elevated Temperatures
Cell Death Induced by Subnanosecond Pulsed Electric Fields at Elevated Temperatures
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DOI：
10.1109/tps.2012.2208202
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发表时间：
2012-10-01
影响因子：
1.5
通讯作者：
Schoenbach, Karl H.
中科院分区：
文献类型：
--
作者：
Camp, J. Thomas;Jing, Yu;Schoenbach, Karl H.
当暴露于亚纳秒高电场脉冲时，肝癌细胞的台盼蓝摄取率（指示细胞死亡）在温度升高到37摄氏度以上时强烈增加。Hepa 1-6细胞暴露于2000个持续时间为200皮秒的脉冲和超过80 kV/cm的电场幅度，当温度在脉冲时间内升高至47 ℃时，诱导几乎30%的细胞死亡。对于37摄氏度及以下的温度，同样暴露于脉冲电场没有显示出任何可测量的影响。即使对于最高升高的温度为47摄氏度，没有发现热效应会导致死亡的曝光时间，这是2000脉冲在重复率为7-9脉冲每秒，在5分钟的顺序。温度对电性能的细胞的影响进行了测量，通过介电谱。发现从这些值得到的膜电压太低，不能在室温下引起电穿孔。然而，随着温度的降低，膜的粘度可能会降低穿孔的阈值，并且与多次脉冲的效果一起，被认为是观察到的细胞高死亡率的原因。这一论点得到了分子动力学模拟的支持，该模拟表明随着温度的升高，孔形成的概率增加。
The rate of trypan blue uptake of liver cancer cells, indicating cell death, when exposed to subnanosecond high electric field pulses, increased strongly when the temperature was raised above 37 degrees C. The exposure of Hepa 1-6 cells to 2000 pulses of 200 picosecond duration and electric field amplitudes exceeding 80 kV/cm induced cell death in almost 30% of the cells when the temperature was increased to 47 degrees C for the time of the pulsing. For temperatures at 37 degrees C and below, the same exposure to pulsed electric fields did not show any measurable effect. Even for the maximum elevated temperature of 47 degrees C, thermal effects were not found to cause fatalities for the time of exposure, which was, for 2000 pulses at a repetition rate of 7-9 pulses per second, on the order of 5 min. The effect of temperature on the electrical properties of the cell was measured by means of dielectric spectroscopy. The membrane voltages derived from these values were found to be too low to cause electroporation at room temperature. However, the reduced viscosity of the membrane with temperature is likely to reduce the threshold for poration, and together with the effect of multiple pulses, is considered to be the cause for the observed high death rate of the cells. This argument is supported by molecular dynamics simulations which show an increased probability for pore formation with temperature.