The administration of hepatocyte growth factor prevents total parenteral nutrition-induced hepatocellular injury in a rat model
The administration of hepatocyte growth factor prevents total parenteral nutrition-induced hepatocellular injury in a rat model
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DOI：
10.1007/s00383-020-04823-9
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发表时间：
2021-01-03
影响因子：
1.8
通讯作者：
Ieiri, Satoshi
中科院分区：
文献类型：
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作者：
Matsukubo, Makoto;Yano, Keisuke;Ieiri, Satoshi
目的全肠外营养（TPN）有时会引起肠外营养相关性肝病（PNALD）。肝细胞生长因子（HGF）是一种有效的肝细胞有丝分裂原，具有抗炎和抗氧化作用。我们的目的是评估HGF对大鼠TPN模型PNALD的影响。方法经右颈静脉置管持续TPN 7 d。所有大鼠分为3组：TPN组（TPN组）、TPN + HGF（低）组（TPN + HGF 0.3 mg/kg/d）和TPN + HGF（高）组（TPN + HGF 1.0 mg/kg/d）。在第7天，收获肝脏并评价组织学、炎性细胞因子和细胞凋亡。结果TPN组肝组织脂滴明显增多，TPN + HGF（低）组和TPN + HGF（高）组脂滴减少。TPN + HGF（低）组和TPN + HGF（高）组的非酒精性脂肪肝活动度评分均显著低于TPN组（p < 0.01）。三组的炎性细胞因子水平无显著差异。TPN + HGF（低）组和TPN + HGF（高）组的caspase-9表达水平与对照组相比显著降低（p < 0.05）。结论静脉注射HGF可剂量依赖性地减轻TPN诱导的肝脂肪变性。
Purpose Total parenteral nutrition (TPN) sometimes induces parenteral nutrition-associated liver disease (PNALD). Hepatocyte growth factor (HGF) acts as a potent hepatocyte mitogen anti-inflammatory and antioxidant actions. We aimed to evaluate the effect of HGF on PNALD in a rat model of TPN. Methods A catheter was placed in the right jugular vein for 7-day continuous TPN. All rats were divided into three groups: TPN alone (TPN group), TPN plus intravenous HGF at 0.3 mg/kg/day [TPN + HGF (low) group], and TPN plus HGF at 1.0 mg/kg/day [TPN + HGF (high) group]. On day 7, livers were harvested and the histology, inflammatory cytokines and apoptosis were evaluated. Results Histologically, lipid droplets were apparent in the TPN group, but decreased in the TPN + HGF (low) and TPN + HGF (high) groups. The histological nonalcoholic fatty liver disease activity scores in the TPN + HGF (low) and TPN + HGF (high) groups were significantly lower than that in the TPN group (p < 0.01). There were no significant differences in the inflammatory cytokine levels of the three groups. The caspase-9 expression levels in the TPN + HGF (low) and TPN + HGF (high) groups were significantly decreased in comparison to that in the control group (p < 0.05). Conclusion The intravenous administration of HGF attenuated hepatic steatosis induced by 7-day TPN dose dependently.