Nucleotide sequence of the marmoset herpesvirus thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide.
Nucleotide sequence of the marmoset herpesvirus thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide.
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狨猴疱疹病毒胸苷激酶基因的核苷酸序列和胸苷激酶多肽的预测氨基酸序列。
DOI：
10.1016/0042-6822(84)90189-2
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
Kit,S
中科院分区：
文献类型：
--
作者：
Otsuka,H;Kit,S
采用双脱氧核苷酸链终止法测定了含绒猴疱疹病毒胸苷激酶基因（tk）整个编码区2549 bp的DNA片段及其侧链序列。从核苷酸序列预测的MarHV胸苷激酶多肽包含376个氨基酸，分子量为41281。测序数据还显示，另一个MarHV基因的编码部分可能在MarHV tk基因停止密码子下游仅292个核苷酸处开始。MarHV tk基因与单纯疱疹病毒（HSV） 1型和2型tk基因的核苷酸序列同源性相对较小。然而，将预测的MarHV胸苷激酶多肽的氨基酸序列与HSV-1和HSV-2胸苷激酶多肽的氨基酸序列进行比较，发现在多肽链的几个区域内存在明显的同源性，但存在中断。在MarHV胸苷激酶多肽的第10 ~ 27个残基和HSV-1和HSV-2胸苷激酶多肽的第49 ~ 66个残基上，氨基酸序列的同源性尤其显著。这些氨基酸残基与线粒体β亚基atp酶、癌基因p21蛋白、腺苷酸激酶和其他核苷酸结合蛋白具有明显的序列同源性。有人提出，这些同源区域是atp酶、p21和腺苷酸激酶中核苷酸结合袋的元件，这提高了MarHV胸苷激酶的氨基酸残基15至25和HSV-1和HSV-2酶的54至64氨基酸残基同样是核苷酸结合位点的可能性。
The nucleotide sequence of a 2549-bp DNA fragment containing the entire coding region of the marmoset herpesvirus (MarHV) thymidine kinase gene (tk) and the flanking sequences was determined by the dideoxynucleotide chain termination method. The MarHV thymidine kinase polypeptide predicted from the nucleotide sequence contained 376 amino acids and had a molecular weight of 41,281. The sequencing data also reveal that the coding portion of another MarHV gene probably begins only 292 nucleotides downstream from the stop codon of the MarHV tk gene. There was relatively little nucleotide sequence homology between the MarHV tk gene and that of the herpes simplex virus (HSV) types 1 and 2 tk genes. Comparisons of the predicted amino acid sequences of the MarHV thymidine kinase polypeptide with that of the HSV-1 and HSV-2 thymidine kinase polypeptides, however, revealed clear, but interrupted, homology within several regions of the polypeptide chains. Amino acid sequence homology was particularly striking at residues 10 to 27 of the MarHV thymidine kinase polypeptide and residues 49 to 66 of the HSV-1 and HSV-2 thymidine kinase polypeptides. These same amino acid residues exhibit noticeable sequence homology to the mitochondrial beta subunit ATPase, oncogene p21 protein, adenylate kinase, and to other nucleotide-binding proteins. It has been proposed that the indicated regions of homology are elements of a nucleotide-binding pocket in ATPase, p21, and adenylate kinase, raising the possibility that amino acid residues 15 to 25 of the MarHV thymidine kinase and 54 to 64 of the HSV-1 and HSV-2 enzymes are likewise parts of nucleotide-binding sites.