Microbial synthesis of gold nanoparticles using the fungus Penicillium brevicompactum and their cytotoxic effects against mouse mayo blast cancer C2C12 cells
Microbial synthesis of gold nanoparticles using the fungus Penicillium brevicompactum and their cytotoxic effects against mouse mayo blast cancer C2C12 cells
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DOI：
10.1007/s00253-011-3556-0
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发表时间：
2011-11-01
影响因子：
5
通讯作者：
Yun, Soon-Il
中科院分区：
文献类型：
--
作者：
Mishra, Amrita;Tripathy, Suraj Kumar;Yun, Soon-Il
微生物、它们的细胞团和活生物质已被用于合成各种金纳米颗粒。生物合成纳米粒子的形状、大小、稳定性以及纯度对于应用目的变得非常重要。在本研究中，金纳米粒子已经从真菌短密青霉菌的上清液、活细胞滤液和生物质中合成。这种真菌生长在马铃薯葡萄糖肉汤中，也被发现可以合成金纳米颗粒。在纯化前、纯化后和在冷藏条件下储存颗粒3个月后，通过Bio-TEM研究颗粒的尺寸。不同的表征技术，如X射线衍射，傅里叶变换红外光谱，紫外-可见光谱已被用于分析的颗粒。还监测了反应参数如pH和金盐浓度的影响，以优化合成的金纳米颗粒的形貌和分散性。5至8的pH范围有利于合成过程，而增加金盐的浓度（超过2 mM）导致形成更大尺寸和聚集的纳米颗粒。此外，在不同的孵育时间间隔（24、48和72小时），使用小鼠马约母细胞癌C2 C12细胞分析了制备的纳米颗粒的细胞毒性性质。在37 ℃、5% CO2加湿环境中，在补充有胎牛血清和抗生素（链青霉素）的Dulbecco改良Eagle培养基中培养细胞。每隔一天补充培养基，并在达到汇合后对细胞进行传代培养。用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴化物法分析细胞活力。
Microorganisms, their cell filtrates, and live biomass have been utilized for synthesizing various gold nanoparticles. The shape, size, stability as well as the purity of the bio synthesized nanoparticles become very essential for application purpose. In the present study, gold nanoparticles have been synthesized from the supernatant, live cell filtrate, and biomass of the fungus Penicillium brevicompactum. The fungus has been grown in potato dextrose broth which is also found to synthesize gold nanoparticles. The size of the particles has been investigated by Bio-TEM before purification, following purification and after storing the particles for 3 months under refrigerated condition. Different characterization techniques like X-ray diffraction, Fourier transform infrared spectroscopy, and UV-visible spectroscopy have been used for analysis of the particles. The effect of reaction parameters such as pH and concentration of gold salt have also been monitored to optimize the morphology and dispersity of the synthesized gold nanoparticles. A pH range of 5 to 8 has favored the synthesis process whereas increasing concentration of gold salt (beyond 2 mM) has resulted in the formation of bigger sized and aggregated nanoparticles. Additionally, the cytotoxic nature of prepared nanoparticles has been analyzed using mouse mayo blast cancer C2C12 cells at different time intervals (24, 48, and 72 h) of incubation period. The cells are cultivated in Dulbecco's modified Eagle's medium supplemented with fetal bovine serum with antibiotics (streptopenicillin) at 37A degrees C in a 5% humidified environment of CO2. The medium has been replenished every other day, and the cells are subcultured after reaching the confluence. The viability of the cells is analyzed with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide method.