Application of Open Source Software Image-J in Microscopic Movement Tracking: Movement Track Reconstruction and Parameter Algorithms
Application of Open Source Software Image-J in Microscopic Movement Tracking: Movement Track Reconstruction and Parameter Algorithms
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DOI：
10.4028/www.scientific.net/amm.66-68.827
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发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
G. Liu
中科院分区：
文献类型：
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作者：
G. Liu
由于运动是微运动细胞的重要特征，因此迫切需要发展微观运动跟踪技术。针对这一需求，举例说明了开源软件Image-J在微观运动跟踪中的应用。运动轨迹重建以圆形和线性运动模式进行。开发和/或解释了实时半径、VCL（曲线速度）、VAP（平均路径速度）、VSL（直线速度）和其他使用原始坐标值的描述性参数的算法。现在可以使用 Image-J 以低成本进行微观运动跟踪。此外，可以轻松控制算法以适应用户的学习目的。
Since movement is an important characteristic of micro-motile cells, the development of microscopic movement tracking techniques is in urgent demand. To meet this need, applications of open source software Image-J in microscopic movement tracking were illustrated with examples. Movement track reconstruction was performed in both circular and linear movement patterns. The algorithms of real time radius, VCL (curvilinear velocity), VAP (average path velocity), VSL (straight line velocity), and other descriptive parameters using original coordinate values were developed and/or explained. Microscopic movement tracking can now be conducted at low cost using Image-J. Moreover, the algorithms can be easily controlled to fit users’ study purpose.