Thermo-responsive polymers and hydrogels in tissue engineering
Thermo-responsive polymers and hydrogels in tissue engineering
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DOI：
10.1515/epoly.2005.5.1.221
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发表时间：
2005-12
期刊：
影响因子：
3.7
通讯作者：
D. Schmaljohann
中科院分区：
文献类型：
--
作者：
D. Schmaljohann
摘要讨论了合成聚合物和水凝胶在组织工程中的各个方面。合成了一系列以N-异丙基丙烯酰胺或N，N-二乙基丙烯酰胺为主链，聚乙二醇为侧链的接枝共聚物，并对其进行了表征。然后将这些聚合物用于制备表面固定化水凝胶。用椭偏仪研究了其在模型衬底上的温度响应行为。使用热响应性聚合物进行体外研究，可以控制表面性质，从而通过温度变化刺激细胞黏附和分离。通过在表面固定的温度响应水凝胶上培养小鼠成纤维细胞来研究细胞响应。研究表明，小鼠成纤维细胞的黏附和分离是温度的函数，这可能与表面性质的变化有关。最后将这一概念扩展到图案化水凝胶的制备，并用成像椭偏仪对其进行了研究。
Abstract Various aspects of synthetic polymers and hydrogels in tissue engineering are discussed. A series of graft copolymers based on either N-isopropylacrylamide or N,N-diethylacrylamide as polymer backbone, and on poly(ethylene glycol) as side chain were prepared and characterized. These polymers were then used for the preparation of surface-immobilized hydrogels. The thermo-responsive behaviour was studied on model substrates by ellipsometry. The use of thermoresponsive polymers for in vitro studies allows the control of surface properties and thus the stimulation of cell adhesion and detachment through temperature changes. The cellular response was investigated by cultivation of mouse fibroblasts on surface-immobilized, thermo-responsive hydrogels. It was demonstrated that mouse fibroblasts adhere and detach as a function of the temperature, which then could be related to the change in surface properties. The concept is finally extended to the preparation of patterned hydrogels, which were studied by imaging ellipsometry.