Effects of miR-101, miR-345 on HBV replication regulation and on the growth of liver cancer cells
Effects of miR-101, miR-345 on HBV replication regulation and on the growth of liver cancer cells
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DOI：
10.3892/ol.2018.9669
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发表时间：
2019-01-01
期刊：
影响因子：
2.9
通讯作者：
Zhang, Lihua
中科院分区：
文献类型：
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作者：
Ding, Aikun;Wang, Cuiyun;Zhang, Lihua
该研究的目的是研究miRNA-101和miRNA-345对HBV复制和肝癌细胞生长的影响。采用qPCR检测miRNA-101和miRNA-345的表达。PCR检测HBV RNA的表达。ELISA法检测HBsAg的表达。MTT法检测BEL-7404细胞增殖情况。BEL-7404 pSUPER.neo-miR-101组和BEL-7404 pSUPER. neo-miR-345组miR-101和miR-345的表达水平显著高于BEL-7404 pSUPER.neo组（P
The aim of the study was to investigate the effects of miRNA-101 and miRNA-345 on HBV replication and liver cancer cell growth. qPCR was performed to detect the expression of miRNA-101 and miRNA-345. The expression of HBV RNA was detected by PCR. The expression of HbsAg was detected using ELISA. BEL-7404 cell line proliferation was detected by MTT assay. The expression levels of miR-101 and miR-345 in BEL-7404 pSUPER.neo-miR-101 group and BEL-7404 pSUPER.neo-miR-345 group were significantly higher than those in BEL-7404 pSUPER.neo group (P