Proviral Loads and Clonal Expansion of HTLV-1-Infected Cells following Vertical Transmission: A 10-Year Follow-Up of Children in Jamaica
Proviral Loads and Clonal Expansion of HTLV-1-Infected Cells following Vertical Transmission: A 10-Year Follow-Up of Children in Jamaica
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DOI：
10.1159/000219384
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发表时间：
2009-01-01
期刊：
影响因子：
4.6
通讯作者：
Okayama, Akihiko
中科院分区：
文献类型：
--
作者：
Umeki, Kazumi;Hisada, Michie;Okayama, Akihiko
目的：很少有研究专门检查前病毒载量（PVL）和克隆进化的人类T淋巴细胞病毒1型（HTLV-1）感染的细胞垂直感染的儿童。研究方法：通过反向长PCR分析牙买加母婴队列研究中3名HTLV-1感染儿童（病例A、B和C）的连续样本（年龄1至16岁）的PVL和外周血单核细胞（PBMC）中HTLV-1感染细胞的克隆扩增。结果如下：病例A的基线PVL（每100，000个PBMC）为260（1岁时），病例B的基线PVL为1，867（3岁时），并且保持恒定超过10年。主要检测到HTLV-1感染细胞克隆扩增的随机模式。相反，病例C，淋巴结病，脂溢性皮炎和反射亢进，显示PVL从1.9岁时的2，819增加到13岁时的13，358，并扩增了2个优势克隆。结论：HTLV-1感染细胞的克隆性扩增是在儿童早期从母亲获得感染后诱导的。应密切监测高PVL和与HTLV-1感染相关的任何体征和症状的青少年。版权所有（C）2009 S. Karger AG，巴塞尔
Objective: Few studies have specifically examined proviral load (PVL) and clonal evolution of human T-lymphotropic virus type 1 (HTLV-1)-infected cells in vertically infected children. Methods: Sequential samples (from ages 1 to 16 years) from 3 HTLV-1-infected children (cases A, B and C) in the Jamaica Mother Infant Cohort Study were analyzed for their PVL and clonal expansion of HTLV-1- infected cells in peripheral blood mononuclear cells (PBMCs) by inverse-long PCR. Results: The baseline PVL (per 100,000 PBMCs) of case A was 260 (at 1 year of age) and of case B it was 1,867 (at 3 years of age), and they remained constant for more than 10 years. Stochastic patterns of clonal expansion of HTLV-1-infected cells were predominately detected. In contrast, case C, who had lymphadenopathy, seborrheic dermatitis and hyperreflexia, showed an increase in PVL from 2,819 at 1.9 years to 13,358 at 13 years of age, and expansion of 2 dominant clones. Conclusion: The clonal expansion of HTLV-1-infected cells is induced in early childhood after infection acquired from their mothers. Youths with high PVL and any signs and symptoms associated with HTLV-1 infection should be closely monitored. Copyright (C) 2009 S. Karger AG, Basel