Burkholderia genome mining for nonribosomal peptide synthetases reveals a great potential for novel siderophores and lipopeptides synthesis.
Burkholderia genome mining for nonribosomal peptide synthetases reveals a great potential for novel siderophores and lipopeptides synthesis.
复制标题
伯克霍尔德氏菌基因组对非核糖体肽合成酶的挖掘揭示了新型铁载体和脂肽合成的巨大潜力。
DOI：
10.1002/mbo3.347
复制
发表时间：
2016-06
期刊：
影响因子：
3.4
通讯作者：
Leclère V
中科院分区：
文献类型：
--
作者：
Esmaeel Q;Pupin M;Kieu NP;Chataigné G;Béchet M;Deravel J;Krier F;Höfte M;Jacques P;Leclère V
伯克霍尔德氏菌是一个重要的属，包括多种物种，包括致病菌株以及促进植物生长的菌株。我们执行了一项全球战略，其中结合了两种相辅相成的办法。第一个是基因组指导的基因组序列的深入分析，第二个是试验指导的实验，以支持在计算机上获得的预测。对伯克霍尔德氏菌属48个无间隙基因组进行的新次级代谢产物的有效筛选显示，总共有161个含有非核糖体肽合成酶（NRPS）的簇，具有合成至少11种新产物的潜力。其中大多数是铁载体或脂肽，这两类产品在生物防治中具有潜在的应用。该策略导致首次在Burkholderia ambifaria AMMD基因组中鉴定了cepaciachelin生物合成的簇，并且在Burkholderia phymatum STM 815基因组中鉴定了对应于新的malleobactin样铁载体（称为phymabactin）的簇。在这两种情况下，当菌株在铁限制条件下生长时产生铁载体。在其他地方，在B. ambifaria AMMD和Burkholderia sp. KJ 006的基因组中检测到抗真菌伯克霍尔德菌素簇。类鼻疽伯克霍尔德菌菌株具有产生一种称为伯克霍霉素的新型脂肽的遗传潜力，该脂肽含有12个单体的肽基部分。由根瘤伯克霍尔德氏菌产生的脂肽混合物将上清液的表面张力从70 mN·m−1降低到27 mN·m−1。非核糖体次级代谢产物的产生似乎与从16 S rRNA序列获得的三个系统发育组有关。此外，基因组挖掘方法为非核糖体合成提供了新的见解，例如在脂肽NRPS中鉴定了双C/E结构域，到目前为止基本上在假单胞菌菌株中发现。
Burkholderia is an important genus encompassing a variety of species, including pathogenic strains as well as strains that promote plant growth. We have carried out a global strategy, which combined two complementary approaches. The first one is genome guided with deep analysis of genome sequences and the second one is assay guided with experiments to support the predictions obtained in silico. This efficient screening for new secondary metabolites, performed on 48 gapless genomes of Burkholderia species, revealed a total of 161 clusters containing nonribosomal peptide synthetases (NRPSs), with the potential to synthesize at least 11 novel products. Most of them are siderophores or lipopeptides, two classes of products with potential application in biocontrol. The strategy led to the identification, for the first time, of the cluster for cepaciachelin biosynthesis in the genome of Burkholderia ambifaria AMMD and a cluster corresponding to a new malleobactin‐like siderophore, called phymabactin, was identified in Burkholderia phymatum STM815 genome. In both cases, the siderophore was produced when the strain was grown in iron‐limited conditions. Elsewhere, the cluster for the antifungal burkholdin was detected in the genome of B. ambifaria AMMD and also Burkholderia sp. KJ006. Burkholderia pseudomallei strains harbor the genetic potential to produce a novel lipopeptide called burkhomycin, containing a peptidyl moiety of 12 monomers. A mixture of lipopeptides produced by Burkholderia rhizoxinica lowered the surface tension of the supernatant from 70 to 27 mN·m−1. The production of nonribosomal secondary metabolites seems related to the three phylogenetic groups obtained from 16S rRNA sequences. Moreover, the genome‐mining approach gave new insights into the nonribosomal synthesis exemplified by the identification of dual C/E domains in lipopeptide NRPSs, up to now essentially found in Pseudomonas strains.