Dynamic expression of microRNAs in M2b polarized macrophages associated with systemic lupus erythematosus
Dynamic expression of microRNAs in M2b polarized macrophages associated with systemic lupus erythematosus
复制标题
系统性红斑狼疮相关的 M2b 极化巨噬细胞中 microRNA 的动态表达
DOI：
10.1016/j.gene.2014.06.065
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发表时间：
2014-09-01
期刊：
影响因子：
3.5
通讯作者：
Xiong, Sidong
中科院分区：
文献类型：
--
作者：
Xiao, Pengli;Dong, Chunsheng;Xiong, Sidong
巨噬细胞极化促进系统性红斑狼疮（SLE）的发生和持续发展。我们先前的研究表明，由活化淋巴细胞衍生的DNA（ALD - DNA）诱导的M2b极化巨噬细胞在SLE疾病的发生和进展中具有关键作用。大量数据表明，微小RNA（miRNAs）是控制巨噬细胞极化的关键调节因子。为了研究SLE中巨噬细胞M2b极化过程中的miRNA调控，在用ALD - DNA刺激6小时和36小时后，对小鼠骨髓来源的巨噬细胞（BMDMs）进行了miRNA微阵列分析。在分析的1111种miRNAs中，超过11%在ALD - DNA触发的巨噬细胞M2b极化过程中出现差异表达。聚类分析揭示了miRNA表达的某些模式，这些模式与ALD - DNA诱导的巨噬细胞M2b极化密切相关。网络结构分析表明，6小时时差异调节的miRNAs的预测功能与炎症反应和疾病显著相关。通过生物网络分析确定，36小时时鉴定出的差异调节的miRNAs与细胞增殖显著相关。在本研究中，首次描述了ALD - DNA诱导巨噬细胞M2b极化过程中的动态miRNA表达模式和网络分析。这些数据不仅有助于更好地理解miRNA介导的巨噬细胞极化，还展示了在SLE患者中靶向miRNAs的未来治疗潜力。（C）2014爱思唯尔有限公司。保留所有权利。
Macrophage polarization contributes to the initiation and perpetuation of systemic lupus erythematosus (SLE). Our previous study demonstrated that M2b polarized macrophages induced by activated lymphocyte-derived DNA (ALD-DNA) have a crucial function in the initiation and progress of SLE disease. Accumulated data suggest that microRNAs (miRNAs) serve as critical regulators to control macrophage polarization. To investigate miRNA regulation during macrophage M2b polarization of SLE, miRNA microarrays of murine bone marrow derived macrophages (BMDMs) were performed following stimulation with ALD-DNA for 6 and 36 h. Over 11% of the 1111 analyzed miRNAs appeared differentially expressed during ALD-DNA triggered macrophage M2b polarization. Cluster analysis revealed certain patterns in miRNA expression that are closely linked to ALD-DNA induced macrophage M2b polarization. Analysis of the network structure showed that the predicted functions of the differentially regulated miRNAs at 6 h are significantly associated with inflammatory response and disease. Differentially regulated miRNAs identified at 36 h were determined to be significantly related to cell proliferation by biological network analysis. In this study, dynamic miRNA expression patterns and network analysis are described for the first time during ALD-DNA induced macrophage M2b polarization. The data not only provide a better understanding of miRNA-mediated macrophage polarization but also demonstrate the future therapeutic potential of targeting miRNAs in SLE patients. (C) 2014 Elsevier B.V. All rights reserved.