Effective permeability of hydrophilic substances through walls of lymph vessels: roles of endothelial barrier
Effective permeability of hydrophilic substances through walls of lymph vessels: roles of endothelial barrier
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DOI：
10.1152/ajpheart.01084.2004
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发表时间：
2005-10-01
影响因子：
4.8
通讯作者：
Ohhashi, T
中科院分区：
文献类型：
--
作者：
Ono, N;Mizuno, R;Ohhashi, T
通过视频显微镜系统，在离体插管大鼠单个淋巴管中评价荧光染料标记的亲水性物质的壁有效渗透性。荧光素钠（NaFl; 332 mol wt）和FITC-葡聚糖（4，400、12，000和71，200 mol wt）给药到淋巴管的管腔内空间中，然后通过氙灯激发。染料荧光强度的变化通过硅增强靶照相机通过适当的滤光片连续测量。通过荧光强度和染料浓度之间的关系计算淋巴管壁中每种染料的净通量。在分离的大鼠淋巴管的管腔内空间中的NaFl和FITC-葡聚糖4，400显著地穿透淋巴管的壁。在离体大鼠淋巴管的管腔内空间中的FITC-葡聚糖12，000略微穿过淋巴管壁，而FITC-葡聚糖71，200不穿透壁。管腔内压力范围从4到8厘米H（2）O没有显着影响本研究中使用的染料的净通量。在将3-[（3-胆酰胺丙基）二甲基铵基]-1-丙磺酸盐施用到淋巴管中后，FITC-葡聚糖4，400和12，000的净通量显著增加，但不是71，200。这些结果表明，小分子亲水性物质（
The wall effective permeability of hydrophilic substances labeled with fluorescent dyes was evaluated in an isolated cannulated rat single lymph vessel through a videomicroscope system. Sodium fluorescein (NaFl; 332 mol wt) and FITC-dextrans ( 4,400, 12,000, and 71,200 mol wt) were administered into the intraluminal space of the lymph vessels and then excited by a Xenon lamp. Changes in the fluorescence intensity of the dyes were continuously measured by a silicon-intensified target camera through appropriate filters. The net flux of each dye in the wall of the lymph vessels was calculated by the relationship between the fluorescence intensity and the concentration of the dyes. NaFl and FITC-dextran 4,400 in the intraluminal space of isolated rat lymph vessels significantly penetrated the wall of the lymph vessels. FITC-dextran 12,000 in the intraluminal space of isolated rat lymph vessels slightly passed through the lymphatic wall, whereas FITC-dextran 71,200 did not penetrate the wall. Intraluminal pressures ranging from 4 to 8 cmH(2)O did not significantly affect the net flux of dyes used in the present study. After administration of 3-[(3- cholamidopropyl) dimethylammonio]-1-propanesulfonate into the lymph vessels, the net flux of FITC-dextran 4,400 and 12,000 but not 71,200 was augmented significantly. These results suggest that small molecular hydrophilic substances (