SCANNING ELECTRON-MICROSCOPY OF COLLAGEN-FIBERS IN INTESTINE
SCANNING ELECTRON-MICROSCOPY OF COLLAGEN-FIBERS IN INTESTINE
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DOI：
10.3109/03008208209160260
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发表时间：
1982-01-01
影响因子：
2.9
通讯作者：
HILTNER, A
中科院分区：
文献类型：
--
作者：
ORBERG, JW;KLEIN, L;HILTNER, A
在扫描电子显微镜[SEM]中观察有和没有机械应力的大鼠肠道的胶原纤维。观察结果与之前通过光学偏光显微镜获得的结果相关联，以进一步了解肠胶原的组织和形态。较大的纤维，.apprx。直径为 4 μm，密集排列成平行的波状阵列。对应力的最初反应是拉直原始纤维。延伸纤维以+30°双轴取向。和-30度。到纵向。这些大纤维表现为亚纤维的集合体。在更高的放大倍数下，较大的纤维似乎陷入了随机取向的小纤维网络中。直径0.2μm。年龄对纤维形态影响的研究表明，大纤维波动的长度在成熟过程中增加，但在老化过程中保持恒定。大纤维和小纤维的直径似乎不随年龄而变化，但更多的 4μm 纤维松散地结合成较大的纤维，这可以在光学显微镜和 SEM 水平上观察到。提出了肠胶原的分层组织。
Collagen fibers of rat intestine were observed with and without mechanical stress in the scanning electron microscope [SEM]. Observations were correlated with the previous results obtained by optical polarized microscopy to provide further insight into the organization and morphology of intestinal collagen. Larger fibers, .apprx. 4 .mu.m in diameter, are densely packed in parallel undulating arrays. The initial response to stress is straightening of the original fibers. The extended fibers are biaxially oriented at +30.degree. and -30.degree. to the longitudinal direction. These large fibers appear as assemblies of subfibers. At higher magnifications, the larger fibers appear to be enmeshed in a network of small randomly oriented fibers .apprx. 0.2 .mu.m in diameter. Study of the effect of age on fiber morphology showed that the length of the large fiber undulations increases during maturation but remains constant during aging. The diameters of large and small fibers appear not to change with age, but more of the 4-.mu.m fibers are loosely associated into larger fibers which can be observed at both the optical microscope and SEM levels. A hierarchical organization of intestinal collagen is proposed.